Purpose: This study investigates the relationships between professional self-concept, patient safety culture, and burnout among cancer hospital nurses. It further aims to identify factors influencing burnout in this population. Methods: This cross-sectional study surveyed 160 nurses at a cancer center. The participants completed self-reported questionnaires on professional self-concept, patient safety culture, and burnout. Results: Pearson's correlation analysis revealed a negative association between patient safety culture and emotional exhaustion and depersonalization. Conversely, a positive association was found between patient safety culture and personal accomplishment and professional self-concept. Regression analysis identified gender (beta=-.18, p=.013), total years of work experience (beta=.27, p=.008), and professional self-concept (beta=-.43, p<.001) as significant factors influencing emotional exhaustion. Similarly, professional self-concept emerged as a significant factor influencing both depersonalization (beta=-.38, p<.001) and personal accomplishment (beta=.54, p<.001). Conclusion: To mitigate emotional exhaustion and depersonalization and enhance personal accomplishment, establishing a program aimed at improving nurses' professional self-concept is essential. The levels of emotional exhaustion and depersonalization among nurses in cancer hospitals are high. Therefore, it may be necessary to develop emotional care programs and provide education on oncology emergencies to prevent and mitigate these issues.