GWAS and WGCNA Analysis Uncover Candidate Genes Associated with Oil Content in Soybean

被引:5
|
作者
Zhao, Xunchao [1 ]
Zhang, Yan [1 ]
Wang, Jie [1 ]
Zhao, Xue [1 ]
Li, Yongguang [1 ]
Teng, Weili [1 ]
Han, Yingpeng [1 ]
Zhan, Yuhang [1 ]
机构
[1] Northeast Agr Univ, Key Lab Soybean Biol, Key Lab Soybean Biol & Breeding Genet, Chinese Minist Educ,Chinese Agr Minist, Harbin 150030, Peoples R China
来源
PLANTS-BASEL | 2024年 / 13卷 / 10期
关键词
soybean; genome-wide association study (GWAS); WGCNA; oil content; QUANTITATIVE TRAIT LOCI; SEED OIL; ACID; QTL; PROTEIN; YIELD; IDENTIFICATION; TRANSCRIPTION; WRINKLED1;
D O I
10.3390/plants13101351
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Soybean vegetable oil is an important source of the human diet. However, the analysis of the genetic mechanism leading to changes in soybean oil content is still incomplete. In this study, a total of 227 soybean materials were applied and analyzed by a genome-wide association study (GWAS). There are 44 quantitative trait nucleotides (QTNs) that were identified as associated with oil content. A total of six, four, and 34 significant QTN loci were identified in Xiangyang, Hulan, and Acheng, respectively. Of those, 26 QTNs overlapped with or were near the known oil content quantitative trait locus (QTL), and 18 new QTNs related to oil content were identified. A total of 594 genes were located near the peak single nucleotide polymorphism (SNP) from three tested environments. These candidate genes exhibited significant enrichment in tropane, piperidine, and pyridine alkaloid biosynthesiss (ko00960), ABC transporters (ko02010), photosynthesis-antenna proteins (ko00196), and betalain biosynthesis (ko00965). Combined with the GWAS and weighted gene co-expression network analysis (WGCNA), four candidate genes (Glyma.18G300100, Glyma.11G221100, Glyma.13G343300, and Glyma.02G166100) that may regulate oil content were identified. In addition, Glyma.18G300100 was divided into two main haplotypes in the studied accessions. The oil content of haplotype 1 is significantly lower than that of haplotype 2. Our research findings provide a theoretical basis for improving the regulatory mechanism of soybean oil content.
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收藏
页数:14
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