CircMEF2C(2, 3) modulates proliferation and adipogenesis of porcine intramuscular preadipocytes by miR-383/671-3p/MEF2C axis

被引:3
作者
Rong, Xiaoyin [1 ,2 ]
Li, Ruixiao [1 ,2 ]
Gong, Tianye [1 ,2 ]
Li, Haonan [1 ,2 ]
Zhao, Xiaolei [1 ,2 ]
Cao, Guoqing [1 ,2 ]
Li, Meng [1 ,2 ]
Li, Bugao [1 ,2 ]
Yang, Yang [1 ,2 ]
Guo, Xiaohong [1 ,2 ]
机构
[1] Shanxi Agr Univ, Coll Anim Sci, Jinzhong 030801, Peoples R China
[2] Key Lab Farm Anim Genet Resources Explorat & Breed, Jinzhong 030801, Peoples R China
基金
中国国家自然科学基金;
关键词
MITOTIC CLONAL EXPANSION; ACTIVATED-RECEPTOR-GAMMA; PPAR-GAMMA; FAT DEPOSITION; DIFFERENTIATION; EXPRESSION; IDENTIFICATION; TRANSCRIPTION; GROWTH; TISSUE;
D O I
10.1016/j.isci.2024.109710
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Circular RNA is a special category of non -coding RNA that has emerged as epigenetic regulator of adipose tissue development. However, the mechanism governing intramuscular adipogenesis of circRNA remains largely uncharted. In this study, circMEF2C(2, 3), looped by MEF2C exons 2 and 3, was identified from the pig MEF2C gene. Expression of circMEF2C(2, 3) is upregulated in early stage of intramuscular adipogenesis and muscular tissue of lean pigs (DLY pig). Subsequently, overexpression or knockdown of circMEF2C(2, 3) reflected that it participates in promoting proliferation and inhibiting adipogenic differentiation in porcine intramuscular preadipocytes and murine C3H10T1/2 cells. Mechanically, circMEF2C(2, 3) competitively combined with miR-383 and miR-671-3p to the 3 0 -UTR of MEF2C , which maintains MEF2C expression in regulating proliferation and adipogenesis. In summary, circMEF2C(2, 3) is a key regulator in the proliferation and adipogenic differentiation of intramuscular adipogenesis, suggesting its potential as a multi -target strategy for adipose development and associated diseases.
引用
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页数:19
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