Screening of stabilizers for LC-MS/MS analysis of clevidipine and its primary metabolite in dog whole blood

被引：10

作者：

Cao, Peng ^{[1
]}

Li, Gao ^{[1
,2
]}

Huang, Ling ^{[1
]}

Zhao, Shouwei ^{[1
]}

Hu, Yang ^{[1
]}

Qin, Lixia ^{[1
]}

Qiu, Lihui ^{[1
]}

Zhu, Wenwen ^{[1
]}

Si, Luqin ^{[1
,2
]}

Huang, Jiangeng ^{[1
,2
]}

机构：

[1] Huazhong Univ Sci & Technol, Sch Pharm, Dept Pharmaceut, Tongji Med Coll, Wuhan 430030, Peoples R China

[2] Huazhong Univ Sci & Technol, Hubei Engn Res Ctr Novel Drug Delivery Syst, Wuhan 430030, Peoples R China

来源：

BIOANALYSIS | 2015年 / 7卷 / 12期

关键词：

TANDEM MASS-SPECTROMETRY; ACTING CALCIUM-ANTAGONIST; LIQUID-CHROMATOGRAPHY; BIOLOGICAL MATRICES; REMIFENTANIL; PLASMA; PRESSURE; DRUG; RAT; PHARMACOKINETICS;

D O I：

10.4155/bio.15.74

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: Clevidipine is an ester-containing antihypertensive agent that undergoes rapid hydrolysis in blood. A reliable stabilizer cocktail containing citric acid and ascorbic acid was established and the LC-MS/MS method was validated for simultaneous determination of clevidipine and its major metabolite in beagle dog whole blood. Results: The stabilizer could nearly completely inhibit the esterase activity. Both analytes were extracted from whole blood by toluene and detected by MS/MS in positive ESI mode. The linearity range was 0.1-100.0 ng/ml for clevidipine and 1.0-1000.0 ng/ml for the primary metabolite. Conclusion: The stabilizer cocktail was able to effectively suppress the activity of esterase in blood. The method was successfully applied to a PK study of clevidipine in beagle dogs.

引用

页码：1457 / 1469

页数：13

共 25 条

[1]

[Anonymous], 2008, MED LETT DRUGS THER, V50, P73

[2]

[Anonymous], 2013, GUID IND BIOAN METH

[3] Factors affecting the stability of drugs and drug metabolites in biological matrices [J].