Oxytocin attenuates neuroinflammation-induced anxiety through restoration of excitation and inhibition balance in the anterior cingulate cortex in mice

被引:7
作者
Shi, Cui-na [1 ]
Wu, Xin-miao [1 ]
Gao, Yu-zhu [1 ]
Ma, Da-qing [2 ,3 ]
Yang, Jian-jun [4 ]
Ji, Mu-huo [1 ]
机构
[1] Nanjing Med Univ, Affiliated Hosp 2, Dept Anesthesiol, Nanjing, Peoples R China
[2] Zhejiang Univ, Childrens Hosp, Natl Clin Res Ctr Child Hlth, Sch Med,Dept Anesthesiol Perioperat & Syst Med Lab, Hangzhou, Peoples R China
[3] Imperial Coll London, Chelsea & Westminster Hosp, Fac Med,Dept Surg & Canc, Div Anaesthet Pain Med & Intens Care, London, England
[4] Zhengzhou Univ, Dept Anesthesiol Pain & Perioperat Med, Affiliated Hosp 1, Zhengzhou, Henan, Peoples R China
基金
中国国家自然科学基金;
关键词
Neuroinflammation; Anxiety; Oxytocin; Excitation/inhibition balance; Anterior cingulate cortex; COGNITIVE IMPAIRMENT; BEHAVIOR; SEPSIS; OSCILLATIONS; SURVIVORS; SYMPTOMS; DISORDER; BRAIN; MODEL;
D O I
10.1016/j.jad.2024.05.144
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Background: Accumulative evidence suggested that the oxytocin system plays a role in socio-emotional disorders, although its role in neuroinflammation-induced anxiety remains unclear. Method: In the present study, anxiety-like behavior was induced in cohorts of animals through repeated lipopolysaccharide (LPS, 0.5 mg/kg, daily, Escherichia coli O55:B5) i.p. injections for seven consecutive days. These different cohorts were subsequently used for anxiety-like behavior assessment with open field test, elevated plus maze, and novelty-suppressed feeding test or for electrophysiology (EEG) recordings of miniature excitatory postsynaptic currents (mEPSCs), miniature inhibitory postsynaptic currents (mIPSCs), or local field potential (LFP) in vivo or ex vivo settings. Samples of the anterior cingulate cortex (ACC) from some cohorts were harvested to conduct immunostaining or western blotting analysis of oxytocin, oxytocin receptor, CamkII, GABA, vGAT, vGLUT2, and c-fos. The dendritic spine density was assessed by Golgi-Cox staining. Results: Repeated LPS injections induced anxiety-like behavior with concurrent decreases of oxytocin, vGLUT2, mEPSC, dendritic spine, c-fos, membrane excitability, and EEG beta and gamma oscillations, but increased oxytocin receptor and vGAT expressions in the ACC; all these changes were ameliorated by oxytocin intranasal or local brain (via cannula) administration. Conclusion: Taken together, our data suggested that oxytocin system may be a therapeutic target for developing treatment to tackle neuroinflammation-induced anxiety.
引用
收藏
页码:341 / 355
页数:15
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