Fucoidan MF4 from Fucus vesiculosus inhibits Lewis lung cancer via STING-TBK1-IRF3 pathway

被引:1
作者
Shi, Chuanqin [1 ,2 ]
Zhao, Shihua [3 ]
Mi, Liyan [4 ]
Niu, Deying [5 ]
Hu, Fanwen [6 ]
Han, Wenwei [7 ]
Li, Bing [1 ,8 ]
机构
[1] Qingdao Univ, Basic Med Coll, Dept Genet & Cell Biol, Qingdao 266071, Peoples R China
[2] Zibo Cent Hosp, Ctr Translat Med, Zibo 255020, Peoples R China
[3] Zibo Cent Hosp, Dept Endocrinol, Zibo 255020, Peoples R China
[4] Qingdao Univ, Affiliated Yantai Yuhuangding Hosp, Dept Neurol, Yantai 264000, Peoples R China
[5] Zibo Cent Hosp, Dept Neurosurg, Zibo 255020, Peoples R China
[6] Jinan Dermatosis Prevent & Contorl Hosp, Departmet Pharm, Jinan 250000, Peoples R China
[7] Univ Hlth & Rehabil Sci, Qingdao Cent Hosp, Qingdao 266071, Peoples R China
[8] Qingdao Univ, Affiliated Hosp, Dept Dermatol, Qingdao 266003, Peoples R China
基金
中国国家自然科学基金; 中国博士后科学基金;
关键词
Fucoidan; Antitumor; STING; POLYSACCHARIDE; IMMUNOTHERAPY; ACTIVATION; IMMUNITY; SENSOR; CELLS;
D O I
10.1016/j.ijbiomac.2024.131336
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Fucoidan, a sulfated polysaccharide of marine origin found in brown algae and sea cucumbers, has been identified as a neuroprotective compound. In this study, a novel fucoidan MF4 was extracted from Fucus vesiculosus and isolated using Q-Sepharose fast-flow ion-exchange chromatography. The physicochemical properties of MF4 were characterized. MF4 is primarily composed of fucose, xylose, galactose, glucose, and mannose in a molar ratio of 12.3: 4.9: 1.1: 1.0: 1.1, with an average molecular weight of 67.7 kDa. Notably, MF4 demonstrated suppression of LLC tumor growth in vivo. RNA-sequencing analysis revealed that MF4 enhanced the expression of type I interferon-associated downstream genes in macrophages. Furthermore, MF4 increased the levels of phosphorylated TBK1 and IRF3 proteins in vitro. By activating the STING-TBK1-IRF3 signaling pathway, MF4 may enhance the antitumor activity of macrophages. Taken together, MF4 has promising potential as an antitumor and immunomodulatory agent.
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页数:11
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共 47 条
[1]   5-Fluorouracil derivatives: a patent review [J].
Alvarez, Pablo ;
Antonio Marchal, Juan ;
Boulaiz, Houria ;
Carrillo, Esmeralda ;
Velez, Celia ;
Rodriguez-Serrano, Fernando ;
Melguizo, Consolacion ;
Prados, Jose ;
Madeddu, Roberto ;
Aranega, Antonia .
EXPERT OPINION ON THERAPEUTIC PATENTS, 2012, 22 (02) :107-123
[2]   Comparison of the immune activation capacities of fucoidan and laminarin extracted from Laminaria japonica [J].
An, Eun-Koung ;
Hwang, Juyoung ;
Kim, So-Jung ;
Park, Hae-Bin ;
Zhang, Wei ;
Ryu, Ja-Hyoung ;
You, SangGuan ;
Jin, Jun-O .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2022, 208 :230-242
[3]   Glycosaminoglycans from a Sea Snake (Lapemis curtus): Extraction, Structural Characterization and Antioxidant Activity [J].
Bai, Mingyue ;
Han, Wenwei ;
Zhao, Xia ;
Wang, Qingchi ;
Gao, Yanyun ;
Deng, Shiming .
MARINE DRUGS, 2018, 16 (05)
[4]   Separation and quantitation of water soluble cellular metabolites by hydrophilic interaction chromatography-tandem mass spectrometry [J].
Bajad, Sunil U. ;
Lu, Wenyun ;
Kimball, Elizabeth H. ;
Yuan, Jie ;
Peterson, Celeste ;
Rabinowitz, Joshua D. .
JOURNAL OF CHROMATOGRAPHY A, 2006, 1125 (01) :76-88
[5]   Treatment duration with immune-based therapies in Cancer: an enigma [J].
Bantia, Shanta ;
Choradia, Nirmal .
JOURNAL FOR IMMUNOTHERAPY OF CANCER, 2018, 6
[6]   TBK1: A key regulator and potential treatment target for interferon positive Sjogren's syndrome, systemic lupus erythematosus and systemic sclerosis [J].
Bodewes, Iris L. A. ;
Huijser, Erika ;
van Helden-Meeuwsen, Cornelia G. ;
Tas, Liselotte ;
Huizinga, Ruth ;
Dalm, Virgil A. S. H. ;
van Hagen, P. Martin ;
Groot, Noortje ;
Kamphuis, Sylvia ;
van Daele, Paul L. A. ;
Versnel, Marjan A. .
JOURNAL OF AUTOIMMUNITY, 2018, 91 :97-102
[7]   Blockade of the programmed death ligand 1 (PD-L1) as potential therapy for anaplastic thyroid cancer [J].
Cantara, Silvia ;
Bertelli, Eugenio ;
Occhini, Rossella ;
Regoli, Mari ;
Brilli, Lucia ;
Pacini, Furio ;
Castagna, Maria Grazia ;
Toti, Paolo .
ENDOCRINE, 2019, 64 (01) :122-129
[8]   Fucoidan-based, tumor-activated nanoplatform for overcoming hypoxia and enhancing photodynamic therapy and antitumor immunity [J].
Chung, Chu-Hung ;
Lu, Kun-Ying ;
Lee, Wei-Cheng ;
Hsu, Wen-Jing ;
Lee, Wen-Fu ;
Dai, Jia-Zih ;
Shueng, Pei-Wei ;
Lin, Cheng-Wei ;
Mi, Fwu-Long .
BIOMATERIALS, 2020, 257
[9]   Specific enhancer selection by IRF3, IRF5 and IRF9 is determined by ISRE half-sites, 5′ and 3′ flanking bases, collaborating transcription factors and the chromatin environment in a combinatorial fashion [J].
Csumita, Maria ;
Csermely, Attila ;
Horvath, Attila ;
Nagy, Gergely ;
Monori, Fanny ;
Goczi, Lorand ;
Orbea, Hans-Acha ;
Reith, Walter ;
Szeles, Lajos .
NUCLEIC ACIDS RESEARCH, 2020, 48 (02) :589-604
[10]   STING-Dependent Cytosolic DNA Sensing Promotes Radiation-Induced Type I Interferon-Dependent Antitumor Immunity in Immunogenic Tumors [J].
Deng, Liufu ;
Liang, Hua ;
Xu, Meng ;
Yang, Xuanming ;
Burnette, Byron ;
Arina, Ainhoa ;
Li, Xiao-Dong ;
Mauceri, Helena ;
Beckett, Michael ;
Darga, Thomas ;
Huang, Xiaona ;
Gajewski, Thomas F. ;
Chen, Zhijian J. ;
Fu, Yang-Xin ;
Weichselbaum, Ralph R. .
IMMUNITY, 2014, 41 (05) :843-852