Fucoidan MF4 from Fucus vesiculosus inhibits Lewis lung cancer via STING-TBK1-IRF3 pathway

被引：1

作者：

Shi, Chuanqin ^{[1
,2
]}

Zhao, Shihua ^{[3
]}

Mi, Liyan ^{[4
]}

Niu, Deying ^{[5
]}

Hu, Fanwen ^{[6
]}

Han, Wenwei ^{[7
]}

Li, Bing ^{[1
,8
]}

机构：

[1] Qingdao Univ, Basic Med Coll, Dept Genet & Cell Biol, Qingdao 266071, Peoples R China

[2] Zibo Cent Hosp, Ctr Translat Med, Zibo 255020, Peoples R China

[3] Zibo Cent Hosp, Dept Endocrinol, Zibo 255020, Peoples R China

[4] Qingdao Univ, Affiliated Yantai Yuhuangding Hosp, Dept Neurol, Yantai 264000, Peoples R China

[5] Zibo Cent Hosp, Dept Neurosurg, Zibo 255020, Peoples R China

[6] Jinan Dermatosis Prevent & Contorl Hosp, Departmet Pharm, Jinan 250000, Peoples R China

[7] Univ Hlth & Rehabil Sci, Qingdao Cent Hosp, Qingdao 266071, Peoples R China

[8] Qingdao Univ, Affiliated Hosp, Dept Dermatol, Qingdao 266003, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2024年 / 267卷

基金：

中国国家自然科学基金; 中国博士后科学基金;

关键词：

Fucoidan; Antitumor; STING; POLYSACCHARIDE; IMMUNOTHERAPY; ACTIVATION; IMMUNITY; SENSOR; CELLS;

D O I：

10.1016/j.ijbiomac.2024.131336

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fucoidan, a sulfated polysaccharide of marine origin found in brown algae and sea cucumbers, has been identified as a neuroprotective compound. In this study, a novel fucoidan MF4 was extracted from Fucus vesiculosus and isolated using Q-Sepharose fast-flow ion-exchange chromatography. The physicochemical properties of MF4 were characterized. MF4 is primarily composed of fucose, xylose, galactose, glucose, and mannose in a molar ratio of 12.3: 4.9: 1.1: 1.0: 1.1, with an average molecular weight of 67.7 kDa. Notably, MF4 demonstrated suppression of LLC tumor growth in vivo. RNA-sequencing analysis revealed that MF4 enhanced the expression of type I interferon-associated downstream genes in macrophages. Furthermore, MF4 increased the levels of phosphorylated TBK1 and IRF3 proteins in vitro. By activating the STING-TBK1-IRF3 signaling pathway, MF4 may enhance the antitumor activity of macrophages. Taken together, MF4 has promising potential as an antitumor and immunomodulatory agent.

引用

页数：11

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