Single-cell-resolved interspecies comparison shows a shared inflammatory axis and a dominant neutrophil-endothelial program in severe COVID-19

被引：3

作者：

Peidli, Stefan ^{[1
,2
,3
,4
,13
]}

Nouailles, Geraldine ^{[1
,2
,5
]}

Wyler, Emanuel ^{[6
]}

Adler, Julia M. ^{[7
]}

Kunder, Sandra ^{[8
]}

Voss, Anne ^{[8
]}

Kazmierski, Julia ^{[9
,10
,14
]}

Pott, Fabian ^{[9
,10
]}

Pennitz, Peter ^{[1
,2
,5
]}

Postmus, Dylan ^{[9
,10
]}

Alves, Luiz Gustavo Teixeira ^{[6
]}

Goffinet, Christine ^{[1
,9
,10
,15
]}

Gruber, Achim D. ^{[1
,8
]}

Thgen, Nils Blu euro ^{[1
,2
,3
,4
]}

Witzenrath, Martin ^{[1
,2
,5
,11
]}

Trimpert, Jakob ^{[1
,7
,12
]}

Landthaler, Markus ^{[4
,6
]}

Praktiknjo, Samantha D. ^{[10
]}

机构：

[1] Humboldt Universitat Berlin, Inst Pathol, Berlin, Germany

[2] Free Univ Berlin, Berlin, Germany

[3] Humboldt Univ, Inst Pathol, Berlin, Germany

[4] Humboldt Univ, Inst Biol, Berlin, Germany

[5] Humboldt Univ, Dept Infect Dis Resp Med & Crit Care, Berlin, Germany

[6] Berlin Inst Med Syst Biol BIMSB, Max Delbruck Ctr Mol Med, Helmholtz Assoc MDC, Berlin, Germany

[7] Free Univ Berlin, Inst Virol, Berlin, Germany

[8] Free Univ Berlin, Inst Vet Pathol, Berlin, Germany

[9] Humboldt Univ, Inst Virol, Berlin, Germany

[10] Charite Univ Med Berlin, Berlin Inst Hlth, Berlin, Germany

[11] German Ctr Lung Res DZL, Berlin, Germany

[12] Kansas State Univ, Coll Vet Med, Dept Diagnost Med Pathobiol, Manhattan, KS USA

[13] European Mol Biol Lab, Heidelberg, Germany

[14] Gladstone Inst Virol, San Francisco, CA USA

[15] Univ Liverpool Liverpool Sch Trop Med, Dept Trop Dis Biol, Liverpool, England

来源：

CELL REPORTS | 2024年 / 43卷 / 06期

关键词：

MODELS;

D O I：

10.1016/j.celrep.2024.114328

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A key issue for research on COVID-19 pathogenesis is the lack of biopsies from patients and of samples at the onset of infection. To overcome these hurdles, hamsters were shown to be useful models for studying this disease. Here, we further leverage the model to molecularly survey the disease progression from timeresolved single -cell RNA sequencing data collected from healthy and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-infected Syrian and Roborovski hamster lungs. We compare our data to human COVID-19 studies, including bronchoalveolar lavage, nasal swab, and postmortem lung tissue, and identify a shared axis of inflammation dominated by macrophages, neutrophils, and endothelial cells, which we show to be transient in Syrian and terminal in Roborovski hamsters. Our data suggest that, following SARS-CoV-2 infection, commitment to a type 1- or type 3 -biased immunity determines moderate versus severe COVID-19 outcomes, respectively.

引用

页数：21

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