Molecular Identification of Genetic Diversity and Population Structure in Moroccan Male Date Palm (Phoenix dactylifera L.) Using Inter-Simple Sequence Repeat, Direct Amplification of Minisatellite DNA, and Simple Sequence Repeat Markers

被引:1
作者
Ibrahimi, Maha [1 ,2 ]
Brhadda, Najiba [2 ]
Ziri, Rabea [2 ]
Fokar, Mohamed [3 ]
Amghar, Ilham [1 ]
Gaboun, Fatima [1 ]
Habach, Aicha [4 ]
Meziani, Reda [5 ]
Elfadile, Jamal [5 ]
Abdelwahd, Rabha [1 ]
Diria, Ghizlane [1 ]
机构
[1] Natl Inst Agr Res INRA, Reg Ctr Agr Res Rabat, Biotechnol Unit, P 6570, Rabat 10101, Morocco
[2] Univ Ibn Tofail, Fac Sci, Lab Plant Anim & Agroind Prod, BP 133, Kenitra 14000, Morocco
[3] Texas Tech Univ, Ctr Biotechnol & Genom, Room 101, Lubbock, TX 79409 USA
[4] Mohammed V Univ, Fac Sci, Lab Microbiol & Mol Biol, BP 8007 NU, Rabat 10000, Morocco
[5] Natl Inst Agr Res INRA, Reg Ctr Agr Res Errachidia, Natl Lab Date Palm Tissues Culture, UR Oasis Syst, BP 2, Errachidia 52000, Morocco
关键词
genetic diversity; Phoenix dactylifera L; ISSR; DAMD; SSR; structure; MICROSATELLITE MARKERS; SSR; POLYMORPHISM; CULTIVARS; GERMPLASM; RAPD; VARIETIES; GENOTYPES; SYSTEM; OASES;
D O I
10.3390/horticulturae10050508
中图分类号
S6 [园艺];
学科分类号
0902 ;
摘要
Understanding genetic diversity and population structure plays a vital role in the efficient use of available material in plant-breeding programs and in germplasm conservation strategies. In the present study, we aim to evaluate the genetic variations and population structure of male date palms from Morocco. The genetic diversity of 100 date palm (Phoenix dactylifera L.) genotypes was investigated using the performance of three types of molecular markers: inter-simple sequence repeats (ISSRs), direct amplification of minisatellite DNA (DAMD), and simple sequence repeats (SSRs). On the basis of their polymorphic information content (PIC) (ISSRs = 0.38; DAMD = 0.4; SSRs = 0.33), effective multiplex ratio (EMR) (ISSRs = 27.34; DAMD = 52.31; SSRs = 22.20), Resolving power Rp (ISSR = 13.81; DAMD = 28.73; SSR = 14.6), and marker index (MI) (ISSRs = 9.22; DAMD = 20.23; SSRs = 7.54) values, all markers used in our study are considered informative markers. Among them, DAMD markers demonstrated slightly higher informativeness compared to ISSR and SSR markers. A total of 216, 438, and 248 bands were, respectively, detected using ISSRs, DAMD, and SSRs, with 95%, 98% and 94% of polymorphism, respectively. The AMOVA results revealed considerable diversity within date palms. The PCOa results showed that males of Tinghir and Errachidia were regrouped into the same cluster, while males of Goulmima were separated into another group. A cluster and structure analysis separated the studied genotypes into three groups. One group comprises genotypes of males from Zagora with some female varieties scattered in this group. The second group includes male genotypes from Goulmima along with accessions of female and male varieties. The third group contains males of Errachidia, Tata and Tinghir populations. The cluster and structure analysis separated the studied genotypes according to their origin.
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页数:19
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