Mechanism Analysis of OsZF8-Mediated Regulation of Rice Resistance to Sheath Blight

被引:1
|
作者
Wang, Yan [1 ]
Wang, Haining [1 ]
Zhang, Liangkun [1 ]
Wang, Yiming [1 ]
Wei, Songhong [1 ]
Wang, Lili [2 ]
机构
[1] Shenyang Agr Univ, Coll Plant Protect, Dept Plant Pathol, Shenyang 110866, Peoples R China
[2] Liaoning Acad Agr Sci, Shenyang 110101, Peoples R China
基金
中国博士后科学基金;
关键词
Rhizoctonia solani; post-transcriptional; zinc finger proteins; transcription factor; TRANSCRIPTION FACTORS; BIOSYNTHESIS; CHOLESTEROL; TOLERANCE; ENHANCE;
D O I
10.3390/ijms25115787
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Transcription factors are key molecules involved in transcriptional and post-transcriptional regulation in plants and play an important regulatory role in resisting biological stress. In this study, we identified a regulatory factor, OsZF8, mediating rice response to Rhizoctonia solani (R. solani) AG1-IA infection. The expression of OsZF8 affects R. solani rice infection. OsZF8 knockout and overexpressed rice plants were constructed, and the phenotypes of mutant and wild-type (WT) plants showed that OsZF8 negatively regulated rice resistance to rice sheath blight. However, it was speculated that OsZF8 plays a regulatory role at the protein level. The interacting protein PRB1 of OsZF8 was screened using the yeast two-hybrid and bimolecular fluorescence complementation test. The results showed that OsZF8 effectively inhibited PRB1-induced cell death in tobacco cells, and molecular docking results showed that PRB1 had a strong binding effect with OsZF8. Further, the binding ability of OsZF8-PRB1 to ergosterol was significantly reduced when compared with the PRB1 protein. These findings provide new insights into elucidating the mechanism of rice resistance to rice sheath blight.
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页数:13
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