Mechanism Analysis of OsZF8-Mediated Regulation of Rice Resistance to Sheath Blight

被引:1
|
作者
Wang, Yan [1 ]
Wang, Haining [1 ]
Zhang, Liangkun [1 ]
Wang, Yiming [1 ]
Wei, Songhong [1 ]
Wang, Lili [2 ]
机构
[1] Shenyang Agr Univ, Coll Plant Protect, Dept Plant Pathol, Shenyang 110866, Peoples R China
[2] Liaoning Acad Agr Sci, Shenyang 110101, Peoples R China
基金
中国博士后科学基金;
关键词
Rhizoctonia solani; post-transcriptional; zinc finger proteins; transcription factor; TRANSCRIPTION FACTORS; BIOSYNTHESIS; CHOLESTEROL; TOLERANCE; ENHANCE;
D O I
10.3390/ijms25115787
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Transcription factors are key molecules involved in transcriptional and post-transcriptional regulation in plants and play an important regulatory role in resisting biological stress. In this study, we identified a regulatory factor, OsZF8, mediating rice response to Rhizoctonia solani (R. solani) AG1-IA infection. The expression of OsZF8 affects R. solani rice infection. OsZF8 knockout and overexpressed rice plants were constructed, and the phenotypes of mutant and wild-type (WT) plants showed that OsZF8 negatively regulated rice resistance to rice sheath blight. However, it was speculated that OsZF8 plays a regulatory role at the protein level. The interacting protein PRB1 of OsZF8 was screened using the yeast two-hybrid and bimolecular fluorescence complementation test. The results showed that OsZF8 effectively inhibited PRB1-induced cell death in tobacco cells, and molecular docking results showed that PRB1 had a strong binding effect with OsZF8. Further, the binding ability of OsZF8-PRB1 to ergosterol was significantly reduced when compared with the PRB1 protein. These findings provide new insights into elucidating the mechanism of rice resistance to rice sheath blight.
引用
收藏
页数:13
相关论文
共 50 条
  • [21] Comparative Field Evaluation and Transcriptome Analysis Reveals that Chromosome Doubling Enhances Sheath Blight Resistance in Rice
    Liu, Sanglin
    Liu, Jiahao
    Wang, Wei
    Yan, Yugang
    Wang, Tianya
    Wu, Jinwen
    Liu, Xiangdong
    Wu, Jian
    Zeng, Yuxiang
    RICE, 2024, 17 (01)
  • [22] Expression of signalling and defence-related genes mediated by over-expression of JERF1, and increased resistance to sheath blight in rice
    Pan, X.
    Li, Y.
    Zhang, H.
    Huang, R.
    Liu, W.
    Ming, J.
    Liu, S.
    Li, X.
    PLANT PATHOLOGY, 2014, 63 (01) : 109 - 116
  • [23] An Overview of Rice QTLs Associated with Disease Resistance to Three Major Rice Diseases: Blast, Sheath Blight, and Bacterial Panicle Blight
    Zarbafi, Seyedeh Soheila
    Ham, Jong Hyun
    AGRONOMY-BASEL, 2019, 9 (04):
  • [24] Evaluation of Seedling Resistance of Rice Germplasm Resources to Sheath Blight (Rhizoctonia solani)
    Chen, B.
    Li, L. -W.
    Lin, Y. -J.
    Wang, Z. -H.
    Lu, G. -D.
    CEREAL RESEARCH COMMUNICATIONS, 2014, 42 (03) : 495 - 502
  • [25] Induction of systemic resistance in rice against sheath blight disease by Pseudomonas fluorescens
    Nandakumar, R
    Babu, S
    Viswanathan, R
    Raguchander, T
    Samiyappan, R
    SOIL BIOLOGY & BIOCHEMISTRY, 2001, 33 (4-5) : 603 - 612
  • [26] Screening and identification of new sources of resistance to sheath blight in wild rice accessions
    Bashyal, B. M.
    Rawat, Kirti
    Singh, Dhiraj
    Krishnan, S. Gopala
    Singh, A. K.
    Singh, N. K.
    Aggarwal, Rashmi
    INDIAN JOURNAL OF GENETICS AND PLANT BREEDING, 2017, 77 (03) : 341 - 347
  • [27] Induction of Systemic Resistance against Sheath Blight in Rice by Different Pseudomonas Isolates
    Elsharkawy, Mohsen Mohamed
    Sakran, Raghda M.
    Ahmad, Abdelmonim Ali
    Behiry, Said, I
    Abdelkhalek, Ahmed
    Hassan, Mohamed M.
    Khedr, Amr Ahmed
    LIFE-BASEL, 2022, 12 (03):
  • [28] Protein Phosphatase 2A Catalytic Subunit PP2A-1 Enhances Rice Resistance to Sheath Blight Disease
    Lin, Qiu Jun
    Chu, Jin
    Kumar, Vikranth
    Yuan, De Peng
    Li, Zhi Min
    Mei, Qiong
    Xuan, Yuan Hu
    FRONTIERS IN GENOME EDITING, 2021, 3
  • [29] bZIP23 interacts with NAC028 to modulate rice resistance to sheath blight disease
    Yuan, De Peng
    Li, Zhuo
    Chen, Huan
    Li, Shuang
    Xuan, Yuan Hu
    Ma, Dianrong
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2023, 672 : 89 - 96
  • [30] Phytochrome B-mediated light signalling enhances rice resistance to saline-alkaline and sheath blight by regulating multiple downstream transcription factors
    Chen, Huan
    Zhou, Tiange
    Wu, Xianxin
    Kumar, Vikranth
    Lan, Xingguo
    Xuan, Yuan Hu
    PLANT BIOTECHNOLOGY JOURNAL, 2025,