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Mechanism Analysis of OsZF8-Mediated Regulation of Rice Resistance to Sheath Blight
被引:1
|作者:
Wang, Yan
[1
]
Wang, Haining
[1
]
Zhang, Liangkun
[1
]
Wang, Yiming
[1
]
Wei, Songhong
[1
]
Wang, Lili
[2
]
机构:
[1] Shenyang Agr Univ, Coll Plant Protect, Dept Plant Pathol, Shenyang 110866, Peoples R China
[2] Liaoning Acad Agr Sci, Shenyang 110101, Peoples R China
基金:
中国博士后科学基金;
关键词:
Rhizoctonia solani;
post-transcriptional;
zinc finger proteins;
transcription factor;
TRANSCRIPTION FACTORS;
BIOSYNTHESIS;
CHOLESTEROL;
TOLERANCE;
ENHANCE;
D O I:
10.3390/ijms25115787
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
Transcription factors are key molecules involved in transcriptional and post-transcriptional regulation in plants and play an important regulatory role in resisting biological stress. In this study, we identified a regulatory factor, OsZF8, mediating rice response to Rhizoctonia solani (R. solani) AG1-IA infection. The expression of OsZF8 affects R. solani rice infection. OsZF8 knockout and overexpressed rice plants were constructed, and the phenotypes of mutant and wild-type (WT) plants showed that OsZF8 negatively regulated rice resistance to rice sheath blight. However, it was speculated that OsZF8 plays a regulatory role at the protein level. The interacting protein PRB1 of OsZF8 was screened using the yeast two-hybrid and bimolecular fluorescence complementation test. The results showed that OsZF8 effectively inhibited PRB1-induced cell death in tobacco cells, and molecular docking results showed that PRB1 had a strong binding effect with OsZF8. Further, the binding ability of OsZF8-PRB1 to ergosterol was significantly reduced when compared with the PRB1 protein. These findings provide new insights into elucidating the mechanism of rice resistance to rice sheath blight.
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页数:13
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