Tear film hTERT and corneal nerve characteristics in dry eye disease

被引：1

作者：

Alotaibi, Sultan ^{[1
]}

Papas, Eric ^{[2
]}

Mobeen, Rabia ^{[2
]}

Ozkan, Jerome ^{[2
]}

Misra, Stuti L. ^{[3
]}

Markoulli, Maria ^{[2
]}

机构：

[1] King Saud Univ, Coll Appl Med Sci, Dept Optometry & Vis Sci, Riyadh, Saudi Arabia

[2] Univ New South Wales, Sch Optometry & Vis Sci, Sydney, Australia

[3] Univ Auckland, Fac Med & Hlth Sci, New Zealand Natl Eye Ctr, Dept Ophthalmol, Auckland, New Zealand

来源：

CLINICAL AND EXPERIMENTAL OPTOMETRY | 2024年

关键词：

Corneal nerves; dry eye; hTERT; ocular surface; tear film; CATALYTIC SUBUNIT; TELOMERASE; CELLS;

D O I：

10.1080/08164622.2024.2354775

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Clinical relevanceThe behaviour of human telomerase reverse transcriptase (hTERT) in tears reflects its role in maintaining the ocular surface homoeostasis, as it is increased after the initial fitting of contact lenses and post-overnight lid closure. BackgroundhTERT has been shown to respond to cellular stress in neurodegenerative diseases and to enhance axonal regeneration after peripheral axotomy in an animal model. This work investigated whether the behaviour of hTERT in the tear film reflects ocular surface inflammation and neuronal changes in the presence of dry eye disease. MethodsFlush tears were collected from 18 participants with dry eye disease (14 females, 4 males, mean age 34.7 +/- 5.2 years) and from 18 healthy participants without dry eye disease (8 females, 10 males, mean age 31.9 +/- 5.8 years). Dry eye disease status was defined using the TFOS DEWS II diagnostic criteria. hTERT levels in tears were measured using enzyme-linked immunosorbent assays. Confocal images were taken at the level of the subbasal nerve plexus at the central cornea and at the inferior whorl, and the densities of corneal immune cells were evaluated as well as corneal nerve morphology metrics using a fully automated technique (University of Manchester, United Kingdom). ResultsIn participants with dry eye disease, hTERT levels were significantly higher compared to controls (median [interquartile range]: 434 [320-600] ng/ml, and 184 [42-390] ng/ml, respectively, p = 0.01). Increased nerve fibre width at the inferior whorl, was seen in those with dry eyes (0.0219 [0.0214-0.0236] mm/mm compared to controls 0.0217 [0.0207 0.0222] p < 0.001), but no significant differences were found in the density of corneal immune cells. ConclusionshTERT levels were elevated in participants with dry eye disease, and this was accompanied by increased nerve thickness in the inferior cornea. The hTERT response may reflect the stress induced to the ocular surface and corneal nerves due to having dry eye disease.

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