Enzymatic fluorescent supramolecular hydrogel with aggregation-induced emission characteristics for sensing alkaline phosphatase

被引：0
作者：
Deng, Yun [1 ]
Fu, Cheng [1 ]
Xu, Aifei [2 ]
He, Rongxiang [1 ]
Lu, Wangting [1 ]
Liu, Min [1 ]
机构：
[1] Jianghan Univ, Inst Interdisciplinary Res,Minist Educ, Coll Photoelect Mat & Technol, Key Lab Optoelect Chem Mat & Devices, Wuhan 430056, Hubei, Peoples R China
[2] Zhengzhou Univ Light Ind, Sch Tobacco Sci & Engn, Zhengzhou 450002, Henan, Peoples R China
来源：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2024年 / 316卷
关键词：
Aggregation -induced emission; Fluorescent supramolecular hydrogel; Alkaline phosphatase; PROBE; MOLECULE; ASSAY;
D O I：
10.1016/j.saa.2024.124334
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
Alkaline phosphatase is an important biomarker for medical diagnosis. An enzymatic fluorescence supramolecular hydrogel with AIE properties was developed and used for sensing alkaline phosphatase in vitro and in living cells. In the presence of ALP, K(TPE)EFYp was partially converted to the hydrogelator K(TPE)EFY and selfassembled into nanofibers to form Hydrogel. With the sol-gel transition and the AIE effect, the fluorescence emission was turned on. The linear concentration range of ALP activity in vitro quantified by this method was determined as 0-3 U/L with a LOD at 0.02 U/L. In addition, cell imaging and serum experiment showed that K (TPE)EFYp could also be used to detect ALP activity in living cells and biological samples.
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页数：7
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