Ternary deep eutectic Solvents-Based Three-Phase partitioning for speciation of Cr (III) and Cr (VI) in edible liquid Oil: A promising method for substituting

In this paper, a novelty ternary deep eutectic solvents was prepared with choline chloride, ethylene glycol and glycerol. The pH value was adjust to develop three-phase for speciation of Cr (III) and Cr (VI) extract and separate in ganoderma lucidum spore oil based on ternary deep eutectic solvents. The results show that DES-4 has a good extraction of Cr (III) and Cr (VI), the sample-extraction ratio was 3:1, Cr (III) was protected by EDTA, all chromium ions in ganoderma lucidum spore oil was extracted by near 100 % in 2 times, Cr (III) and Cr (VI) was well separated by saturated buffer solution of KH2PO4/H3PO4 (pH = 4.5). The LOD is 0.031 mg/kg. The RSD is 4.1 %. The recovery between 85.2 % and 111.5 %. This method was successfully applied to detect speciation chromium in other edible liquid oil.