Peripheral effects induced in BALB/c mice infected with DENV by the intracerebral route

被引：20

作者：

Oliveira, E. R. A. ^{[1
]}

Amorim, J. F. S. ^{[1
]}

Paes, M. V. ^{[1
]}

Azevedo, A. S. ^{[1
]}

Goncalves, A. J. S. ^{[1
]}

Costa, S. M. ^{[1
]}

Mantuano-Barradas, M. ^{[1
]}

Povoa, T. F. ^{[1
]}

de Meis, J. ^{[2
]}

Basilio-de-Oliveira, C. A. ^{[3
]}

Nogueira, A. C. M. A. ^{[1
]}

Alves, A. M. B. ^{[1
]}

机构：

[1] Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Lab Biotechnol & Physiol Viral Infect, Rio De Janeiro, Brazil

[2] Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Lab Thymus Res, Rio De Janeiro, Brazil

[3] Fed Univ State Rio de Janeiro UNIRIO, Hosp Gaffree Guinle, Pathol Anat, Rio De Janeiro, RJ, Brazil

来源：

VIROLOGY | 2016年 / 489卷

关键词：

Dengue; Mouse model; Intracerebral infection; Immune response; Central nervous system; DENGUE VIRUS-INFECTION; CENTRAL-NERVOUS-SYSTEM; NEUROLOGIC MANIFESTATIONS; HEMORRHAGIC-FEVER; MOUSE MODELS; LIVER; CELLS; INVOLVEMENT; GATEWAY; BURDEN;

D O I：

10.1016/j.virol.2015.12.006

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The lack of an immunocompetent animal model for dengue mimicking the disease in humans is a limitation for advances in this field. Inoculation by intracerebral route of neuroadapted dengue strains in mice is normally lethal and provides a straightforward readout parameter for vaccine testing. However, systemic effects of infection and the immune response elicited in this model remain poorly described. In the present work, BALB/c mice infected by the intracerebral route with neuroadapted DENV2 exhibited several evidences of systemic involvement. DENV-inoculated mice presented virus infective particles in the brain followed by viremia, especially in late stages of infection. Infection induced cellular and humoral responses, with presence of activated T cells in spleen and blood, lymphocyte infiltration and tissue damages in brain and liver, and an increase in serum levels of some pro-inflammatory cytokines. Data highlighted an interplay between the central nervous system commitment and peripheral effects under this experimental condition. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：95 / 107

页数：13

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