Cross-Reactive Antibodies to the NS1 Protein of Omsk Hemorrhagic Fever Virus Are Absent in the Sera of Patients with Tick-Borne Encephalitis

被引:0
作者
Kravchuk, Bogdana I. [1 ]
Khlusevich, Yana A. [1 ]
Chicherina, Galina S. [2 ]
Yakimenko, Valeriy V. [3 ]
Krasnova, Elena I. [4 ]
Tikunova, Nina N. [1 ]
Matveev, Andrey L. [1 ]
机构
[1] Russian Acad Sci, Inst Chem Biol & Fundamental Med, Siberian Branch, Novosibirsk 630090, Russia
[2] Russian Acad Sci, Inst Systemat & Ecol Anim, Siberian Branch, Novosibirsk 630091, Russia
[3] Omsk Res Inst Nat Foci Infect, Omsk 644080, Russia
[4] Higher Educ Russian Univ Med, Fed State Budgetary Educ Inst, Minist Hlth Russian Federat, Novosibirsk 630091, Russia
来源
VIRUSES-BASEL | 2024年 / 16卷 / 07期
基金
俄罗斯科学基金会;
关键词
NS1; protein; Omsk hemorrhagic fever virus; OHFV; tick-borne encephalitis virus; TBEV; flaviviruses; flavivirus diagnosis; FLAVIVIRUS; SECRETION; INFECTION; KINETICS; VACCINE;
D O I
10.3390/v16071032
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Omsk hemorrhagic fever virus (OHFV) is a member of the tick-borne encephalitis virus (TBEV) complex of the Flaviviridae family. Currently, there are no data on the cross-reactivity of antibodies to the NS1 proteins of OHFV and TBEV. Such data are of major interest for monitoring viral encephalitis of unknown etiology due to the increasing geographical distribution of OHFV. In this study, a recombinant OHFV NS1 protein was produced using the Escherichia coli expression system and purified. The recombinant OHFV NS1 protein was recognized by specific mice immune ascetic fluids to the native OHFV NS1 protein. A Western blot analysis and ELISA of the recombinant NS1 proteins of OHFV and TBEV were used to study the cross-reactivity of antibodies from immune ascites fluid obtained from OHFV-infected mice and mAbs against TBEV NS1. Anti-TBEV NS1 mouse monoclonal antibodies (mAbs) have been shown to not be cross-reactive to the OHFV NS1 protein. Sera from patients with confirmed tick-borne encephalitis (TBE) were examined by ELISA using recombinant OHFV NS1 and TBEV NS1 proteins as antigens. It was shown for the first time that cross-reactive antibodies to the OHFV NS1 protein were not detected in the sera of TBE patients, whereas the sera contained antibodies to the TBEV NS1 protein.
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页数:15
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共 34 条
  • [1] Structure-guided insights on the role of NS1 in flavivirus infection
    Akey, David L.
    Brown, W. Clay
    Jose, Joyce
    Kuhn, Richard J.
    Smith, Janet L.
    [J]. BIOESSAYS, 2015, 37 (05) : 489 - 494
  • [2] Flavivirus NS1 Structures Reveal Surfaces for Associations with Membranes and the Immune System
    Akey, David L.
    Brown, W. Clay
    Dutta, Somnath
    Konwerski, Jamie
    Jose, Joyce
    Jurkiw, Thomas J.
    DelProposto, James
    Ogata, Craig M.
    Skiniotis, Georgios
    Kuhn, Richard J.
    Smith, Janet L.
    [J]. SCIENCE, 2014, 343 (6173) : 881 - 885
  • [3] Tick-borne encephalitis nonstructural protein NS1 expressed in E. coli retains immunological properties of the native protein
    Andrey, Matveev
    Yana, Khlusevich
    Olga, Golota
    Bogdana, Kravchuk
    Sergey, Tkachev
    Lyudmila, Emelyanova
    Nina, Tikunova
    [J]. PROTEIN EXPRESSION AND PURIFICATION, 2022, 191
  • [4] Extended surface for membrane association in Zika virus NS1 structure
    Brown, W. Clay
    Akey, David L.
    Konwerski, Jamie R.
    Tarrasch, Jeffrey T.
    Skiniotis, Georgios
    Kuhn, Richard J.
    Smith, Janet L.
    [J]. NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2016, 23 (09) : 865 - 867
  • [5] Flavivirus NS1 and Its Potential in Vaccine Development
    Carpio, Kassandra L.
    Barrett, Alan D. T.
    [J]. VACCINES, 2021, 9 (06)
  • [6] Evaluation of the European tick-borne encephalitis vaccine against Omsk hemorrhagic fever virus
    Chidumayo, Nozyechi N.
    Yoshii, Kentaro
    Kariwa, Hiroaki
    [J]. MICROBIOLOGY AND IMMUNOLOGY, 2014, 58 (02) : 112 - 118
  • [7] Flavivirus infection-A review of immunopathogenesis, immunological response, and immunodiagnosis
    Chong, Hui Ying
    Leow, Chiuan Yee
    Majeed, Abu Bakar Abdul
    Leow, Chiuan Herng
    [J]. VIRUS RESEARCH, 2019, 274
  • [8] Defining the levels of secreted non-structural protein NS1 after west nile virus infection in cell culture and mice
    Chung, Kyung Min
    Diamond, Michael S.
    [J]. JOURNAL OF MEDICAL VIROLOGY, 2008, 80 (03) : 547 - 556
  • [9] THE NS1 PROTEIN OF TICK-BORNE ENCEPHALITIS-VIRUS FORMS MULTIMERIC SPECIES UPON SECRETION FROM THE HOST-CELL
    CROOKS, AJ
    LEE, JM
    EASTERBROOK, LM
    TIMOFEEV, AV
    STEPHENSON, JR
    [J]. JOURNAL OF GENERAL VIROLOGY, 1994, 75 : 3453 - 3460
  • [10] Challenges in Direct Detection of Flaviviruses: A Review
    Dias, Bruna de Paula
    Barbosa, Camila Cavadas
    Ferreira, Cyntia Silva
    dos Santos, Samara Mayra Soares Alves
    Arrieta, Orlando Alfredo Pineda
    Malta, Wellington Carvalho
    Gomes, Maria Laura Maximiano Dias
    Silva, Mariela Alves e
    Fonseca, Julia de Matos
    Borges, Lysandro Pinto
    Silva, Breno de Mello
    [J]. PATHOGENS, 2023, 12 (05):