Development of an Enzyme-Linked Immunosorbent Assay (ELISA) as a tool to detect NS1 of dengue virus serotype 2 in female Aedes aegypti eggs for the surveillance of dengue fever transmission

被引：3

作者：

Argotte-Ramos, Rocio ^{[1
]}

Cime-Castillo, Jorge ^{[1
]}

Vargas, Valeria ^{[1
]}

Lanz-Mendoza, Humberto ^{[1
]}

Rodriguez, Mario H. ^{[1
]}

Rodriguez, Maria Carmen ^{[1
]}

机构：

[1] Inst Nacl Salud Publ, Ctr Invest Enfermedades Infecciosas, Av Univ 655, Cuernavaca 62100, Morelos, Mexico

来源：

HELIYON | 2024年 / 10卷 / 08期

关键词：

Aedes aegypti; Dengue virus; Surveillance; ELISA; NS1; Egg homogenates; NATURAL VERTICAL TRANSMISSION; TRANS-OVARIAL TRANSMISSION; MOSQUITOS; ALBOPICTUS; ANTIGEN; STRAINS; PROTEIN; GROWTH;

D O I：

10.1016/j.heliyon.2024.e29329

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Dengue is a significant disease transmitted by Aedes mosquitoes in the tropics and subtropics worldwide. The disease is caused by four virus (DENV) serotypes and is transmitted to humans by female Aedes aegypti mosquito bites infected with the virus and vertically to their progeny. Current strategies to control dengue transmission focus on the vector. In this study, we describe an indirect Enzyme-Linked Immunosorbent Assay (ELISA), using a monoclonal antibody against the non-structural dengue virus protein 1 (NS1), to detect DENV2 in Ae. aegypti eggs. The assay detects NS1 in eggs homogenates with 87.5% sensitivity and 75.0% specificity and it is proposed as a tool for the routine entomovirological surveillance of DENV 2 in field mosquito populations.

引用

页数：8

共 32 条

[1] Detection of recombinant dengue virus 2 NS1 protein in Aedes aegypti mosquitoes using commercial Dengue NS1 ELISA kit [J].

Abraham, Philip ;

Sankari, T. ;

Kumar, N. ;

Kumar, Ashwani .

JOURNAL OF VECTOR BORNE DISEASES, 2022, 59 (01) :98-101

[2] Dengue NS1 antigen kit shows high sensitivity for detection of recombinant dengue virus-2 NS1 antigen spiked with Aedes aegypti mosquitoes [J].

Abraham, Philip Raj ;

Bharathy, R. ;

Kumar N, Pradeep ;

Kumar, Ashwani .

SCIENTIFIC REPORTS, 2021, 11 (01)

[3] Development and evaluation of an indirect ELISA for detection of Teladorsagia circumcincta infection in sheep [J].

Aliabadi, Jalal ;

Rakhshandehroo, Ehsan ;

Yektaseresht, Azadeh .

BMC VETERINARY RESEARCH, 2021, 17 (01)

[4] Serotype-specific detection of dengue viruses in a nonstructural protein 1-based enzyme-linked immunosorbent assay validated with a multi-national cohort [J].

Bosch, Irene ;

Reddy, Ankita ;

de Puig, Helena ;

Ludert, Juan E. ;

Perdomo-Celis, Federico ;

Narvaez, Carlos F. ;

Versiani, Alice ;

Fandos, Diana ;

Nogueira, Mauricio L. ;

Singla, Mohit ;

Lodha, Rakesh ;

Medigeshi, Guruprasad R. ;

Lorenzana, Ivette ;

Ralde, Hugo Vicente ;

Gelvez-Ramirez, Margarita ;

Villar, Luis A. ;

Hiley, Megan ;

Mendoza, Laura ;

Salcedo, Nol ;

Herrera, Bobby Brooke ;

Gehrke, Lee .

PLOS NEGLECTED TROPICAL DISEASES, 2020, 14 (06) :1-15

[5] Vertical Transmission of Key West Dengue-1 Virus by Aedes aegypti and Aedes albopictus (Diptera: Culicidae) Mosquitoes From Florida [J].

Buckner, Eva A. ;

Alto, Barry W. ;

Lounibos, L. Philip .

JOURNAL OF MEDICAL ENTOMOLOGY, 2013, 50 (06) :1291-1297

[6] Detection of NS1 protein from dengue virus in excreta and homogenates of wild-caught Aedes aegypti mosquitoes using monoclonal antibodies [J].

Carmen Rodriguez, Maria ;

Cime-Castillo, Jorge ;

Argotte-Ramos, Rocio ;

Vargas, Valeria ;

Correa-Morales, Fabian ;

Sanchez-Tejeda, Gustavo ;

Lanz-Mendoza, Humberto .

PATHOGENS AND DISEASE, 2022, 80 (01)

[7] Nonstructural Protein 1-Specific Immunoglobulin M and G Antibody Capture Enzyme-Linked Immunosorbent Assays in Diagnosis of Flaviviral Infections in Humans [J].

Chao, Day-Yu ;

Galula, Jedhan Ucat ;

Shen, Wen-Fan ;

Davis, Brent S. ;

Chang, Gwong-Jen J. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2015, 53 (02) :557-566

[8] Monitoring of dengue viruses in field-caught Aedes aegypti and Aedes albopictus mosquitoes by a type-specific polymerase chain reaction and cycle sequencing [J].

Chow, VTK ;

Chan, YC ;

Yong, R ;

Lee, KM ;

Lim, LK ;

Chung, YK ;

Lam-Phua, SG ;

Tan, BT .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1998, 58 (05) :578-586

[9]

Clements A N., 2012, Biol. mosquitoes, V3

[10] Transovarial transmission of DENV in Aedes aegypti in the Amazon basin: a local model of xenomonitoring [J].

da Costa, Cristiano Fernandes ;

dos Passos, Ricardo Augusto ;

Pereira Lima, Jose Bento ;

Roque, Rosemary Aparecida ;

Sampaio, Vanderson de Souza ;

Campolina, Thais Bonifacio ;

Costa Secundino, Nagila Francinete ;

Paolucci Pimenta, Paulo Filemon .

PARASITES & VECTORS, 2017, 10

← 1 2 3 4 →