Genome Characteristics of the Endophytic Fungus Talaromyces sp. DC2 Isolated from Catharanthus roseus (L.) G. Don

被引:1
|
作者
Quan, Nguyen Duc [1 ]
Nguyen, Ngoc-Lan [1 ,2 ]
Giang, Tran Thi Huong [1 ]
Ngan, Nguyen Thi Thanh [1 ,2 ]
Hien, Nguyen Thanh [1 ]
Tung, Nguyen Van [1 ,2 ]
Trang, Nguyen Hoang Thanh [1 ]
Lien, Nguyen Thi Kim [1 ,2 ]
Nguyen, Huy Hoang [1 ,2 ]
机构
[1] Vietnam Acad Sci & Technol, Inst Genome Res, 18 Hoang Quoc Viet Str, Hanoi 100000, Vietnam
[2] Grad Univ Sci & Technol, Vietnam Acad Sci & Technol, Dept Biotechnol, 18 Hoang Quoc Viet Str, Cau Giay 100000, Hanoi, Vietnam
关键词
Talaromyces; DC2; genome sequencing; endophytic fungus; Catharanthus roseus; secondary metabolites; plant cell wall degradation; DDC gene; BIOSYNTHESIS; PENICILLIUM; IDENTIFICATION; DECARBOXYLASE; DEGRADATION;
D O I
10.3390/jof10050352
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Talaromyces sp. DC2 is an endophytic fungus that was isolated from the stem of Catharanthus roseus (L.) G. Don in Hanoi, Vietnam and is capable of producing vinca alkaloids. This study utilizes the PacBio Sequel technology to completely sequence the whole genome of Talaromyces sp. DC2The genome study revealed that DC2 contains a total of 34.58 Mb spanned by 156 contigs, with a GC content of 46.5%. The identification and prediction of functional protein-coding genes, tRNA, and rRNA were comprehensively predicted and highly annotated using various BLAST databases, including non-redundant (Nr) protein sequence, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Clusters of Orthologous Groups (COG), and Carbohydrate-Active Enzymes (CAZy) databases. The genome of DC2 has a total of 149, 227, 65, 153, 53, and 6 genes responsible for cellulose, hemicellulose, lignin, pectin, chitin, starch, and inulin degradation, respectively. The Antibiotics and Secondary Metabolites Analysis Shell (AntiSMASH) analyses revealed that strain DC2 possesses 20 biosynthetic gene clusters responsible for producing secondary metabolites. The strain DC2 has also been found to harbor the DDC gene encoding aromatic L-amino acid decarboxylase enzyme. Conclusively, this study has provided a comprehensive understanding of the processes involved in secondary metabolites and the ability of the Talaromyces sp. DC2 strain to degrade plant cell walls.
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页数:18
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