A Mouse Model for Eosinophilic Esophagitis (EoE)

Eosinophilic esophagitis (EoE) is an emerging chronic T helper type 2 (Th2)-associated, allergic, and immune-mediated disease, characterized histologically by eosinophil-predominant mucosal inflammation and clinically by esophageal dysfunction. Over the past years, the prevalence of EoE has dramatically increased globally. Until recently, most studies of EoE focused on using human biopsies, which are also used for diagnostic purposes, or esophageal epithelial cell lines, which led to major advances in the understanding of EoE. Despite this, a robust mouse model that mimics human disease is still crucial for both understanding disease pathogenesis and as a preclinical model for testing future therapeutics. Herein, we describe a highly reproducible and robust model of EoE that can be performed using wild-type mice by ear sensitization with oxazolone (OXA) followed by intraesophageal challenges. Experimental EoE elicited by OXA mimics the main histopathological features of human EoE, including intraepithelial eosinophilia, epithelial and lamina propria thickening, basal cell hyperplasia, and fibrosis. (c) 2024 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol: Induction of EoE in mice using oxazoloneSupport Protocol 1: Preparing the mouse esophagus for histological analysisSupport Protocol 2: Assessment of epithelial and lamina propria thickness using H&E stainingSupport Protocol 3: Assessment of eosinophilic infiltration using anti-MBP and basal cell proliferation using anti-Ki-67 stainingSupport Protocol 4: Flow cytometry of mouse esophageal samplesSupport Protocol 5: ELISA on protein lysates of esophageal samples