Iron binding to human heavy-chain ferritin

被引:70
作者
Pozzi, Cecilia [1 ]
Di Pisa, Flavio [1 ]
Bernacchioni, Caterina [2 ,3 ]
Ciambellotti, Silvia [2 ,3 ]
Turano, Paola [2 ,3 ]
Mangani, Stefano [1 ,3 ]
机构
[1] Univ Siena, Dipartimento Biotecnol Chim & Farm, I-53100 Siena, Italy
[2] Univ Florence, Dipartimento Chim, I-50019 Florence, Italy
[3] Univ Florence, CERM, I-50019 Florence, Italy
来源
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2015年 / 71卷
关键词
ferritin; human heavy chain; iron; mechanism; HUMAN H-CHAIN; ISOTHERMAL TITRATION CALORIMETRY; FERROXIDASE SITE; PYROCOCCUS-FURIOSUS; CYTOCHROME P450CAM; CRYSTAL-STRUCTURES; PROTEIN NANOCAGES; ESCHERICHIA-COLI; MECHANISM; DIOXYGEN;
D O I
10.1107/S1399004715013073
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Maxi-ferritins are ubiquitous iron-storage proteins with a common cage architecture made up of 24 identical subunits of five alpha-helices that drive iron biomineralization through catalytic iron(II) oxidation occurring at oxidoreductase sites (OS). Structures of iron-bound human H ferritin were solved at high resolution by freezing ferritin crystals at different time intervals after exposure to a ferrous salt. Multiple binding sites were identified that define the iron path from the entry ion channels to the oxidoreductase sites. Similar data are available for another vertebrate ferritin: the M protein from Rana catesbeiana. A comparative analysis of the iron sites in the two proteins identifies new reaction intermediates and underlines clear differences in the pattern of ligands that define the additional iron sites that precede the oxidoreductase binding sites along this path. Stopped-flow kinetics assays revealed that human H ferritin has different levels of activity compared with its R. catesbeiana counterpart. The role of the different pattern of transient iron-binding sites in the OS is discussed with respect to the observed differences in activity across the species.
引用
收藏
页码:1909 / 1920
页数:12
相关论文
共 46 条
  • [1] Ferritins: A family of molecules for iron storage, antioxidation and more
    Arosio, Paolo
    Ingrassia, Rosaria
    Cavadini, Patrizia
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2009, 1790 (07): : 589 - 599
  • [2] Is His54 a gating residue for the ferritin ferroxidase site?
    Bernacchioni, Caterina
    Ciambellotti, Silvia
    Theil, Elizabeth C.
    Turano, Paola
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2015, 1854 (09): : 1118 - 1122
  • [3] Loop Electrostatics Modulates the Intersubunit Interactions in Ferritin
    Bernacchioni, Caterina
    Ghini, Veronica
    Pozzi, Cecilia
    Di Pisa, Flavio
    Theil, Elizabeth C.
    Turano, Paola
    [J]. ACS CHEMICAL BIOLOGY, 2014, 9 (11) : 2517 - 2525
  • [4] Structural Insights into the Ferroxidase Site of Ferritins from Higher Eukaryotes
    Bertini, Ivano
    Lalli, Daniela
    Mangani, Stefano
    Pozzi, Cecilia
    Rosa, Camilla
    Theil, Elizabeth C.
    Turano, Paola
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2012, 134 (14) : 6169 - 6176
  • [5] Functionality of the Three-Site Ferroxidase Center of Escherichia coli Bacterial Ferritin (EcFtnA)
    Bou-Abdallah, F.
    Yang, H.
    Awomolo, A.
    Cooper, B.
    Woodhall, M. R.
    Andrews, S. C.
    Chasteen, N. D.
    [J]. BIOCHEMISTRY, 2014, 53 (03) : 483 - 495
  • [6] Defining metal ion inhibitor interactions with recombinant human H- and L-chain ferritins and site-directed variants: an isothermal titration calorimetry study
    Bou-Abdallah, F
    Arosio, P
    Levi, S
    Janus-Chandler, C
    Chasteen, ND
    [J]. JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY, 2003, 8 (04): : 489 - 497
  • [7] μ-1,2-peroxobridged di-iron(III) dimer formation in human H-chain ferritin
    Bou-Abdallah, F
    Papaefthymiou, GC
    Scheswohl, DM
    Stanga, SD
    Arosio, P
    Chasteen, ND
    [J]. BIOCHEMICAL JOURNAL, 2002, 364 : 57 - 63
  • [8] Ferrous ion binding to recombinant human H-chain ferritin. An isothermal titration calorimetry study
    Bou-Abdallah, F
    Arosio, P
    Santambrogio, P
    Yang, X
    Janus-Chandler, C
    Chasteen, ND
    [J]. BIOCHEMISTRY, 2002, 41 (37) : 11184 - 11191
  • [9] Mechanisms of iron mineralization in ferritins: one size does not fit all
    Bradley, Justin M.
    Moore, Geoffrey R.
    Le Brun, Nick E.
    [J]. JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY, 2014, 19 (06): : 775 - 785
  • [10] X-ray structures of ferritins and related proteins
    Crichton, Robert R.
    Declercq, Jean-Paul
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2010, 1800 (08): : 706 - 718