Transcriptomic Analysis of Green Leaf Plants and White-Green Leaf Mutants in Haworthia cooperi var. pilifera

被引:1
|
作者
Li, Peiling [1 ]
Ren, Maofei [1 ]
Chen, Juanjuan [1 ]
Yue, Jianhua [1 ]
Liu, Songhu [1 ]
Zhu, Qingsong [1 ]
Wang, Zhiyong [1 ]
机构
[1] Xinyang Agr & Forestry Univ, Coll Hort, Xinyang 464000, Peoples R China
基金
中国国家自然科学基金;
关键词
white-green leaf mutant; Haworthia cooperi var. pilifera; transcriptome analysis; leaf color; GENE; EXPRESSION; BIOSYNTHESIS; PROTEIN; OVEREXPRESSION; ACCUMULATION; ANTHOCYANIN; RESISTANCE; CHELATASE; SYNTHASE;
D O I
10.3390/genes15050608
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Haworthia cooperi var. pilifera is a succulent plant with ornamental value. The white-green leaf mutant (wl) showed a significant difference in leaf color from the wild-type plant (WT). In this study, we integrated the transcriptomes of wl and WT plants to screen differentially expressed genes related to leaf color variation. The results of transcriptome analysis showed that 84,163 unigenes were obtained after de novo assembly and the NR database annotated the largest number of unigenes, which accounted for 57.13%, followed by NT (43.02%), GO (39.84%), Swiss-Prot (39.25%), KEGG (36.06%), and COG (24.88%). Our finding showed that 2586 genes were differentially expressed in the two samples, including 1996 down-regulated genes and 590 up-regulated genes. GO analysis predicted that these differentially expressed genes (DEGs) participate in 12 cellular components, 20 biological processes, and 13 molecular function terms and KEGG analysis showed that metabolic pathways, plant-pathogen interaction, glycerophospholipid metabolism, endocytosis, plant hormone signal transduction, and ether lipid metabolism were enriched among all identified pathways. Through functional enrichment analysis of DEGs, we found that they were involved in chloroplast division and the biosynthesis of plant pigments, including chlorophyll, carotenoids, anthocyanin, and transcription factor families, which might be related to the formation mechanism of leaf color. Taken together, these results present insights into the difference in gene expression characteristics in leaves between WT and wl mutants and provide a new insight for breeding colorful leaf phenotypes in succulent plants.
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页数:13
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