The anti-neuroinflammatory potential of himalayan high-altitude medicinal plant secondary metabolites using SK-N-SH neuronal cell lines

被引：0

作者：

Kulkarni, Amod ^{[1
]}

Sharma, Rajni Kant ^{[2
]}

Bhavna ^{[3
]}

Singh, Sushila ^{[2
]}

Prashu ^{[4
]}

Mohan, Man ^{[5
]}

Blackhurst, Dee M. ^{[6
]}

Davids, Lester M. ^{[7
]}

Kellaway, Lauriston ^{[7
]}

机构：

[1] Univ Cape Town, Dept Human Biol, Div Neurosci, ZA-7700 Cape Town, South Africa

[2] CCS Haryana Agr Univ, Dept Chem, Hisar 125004, Haryana, India

[3] Govt Girls Sr Secondary Sch, Thanesar 136118, Haryana, India

[4] Maharishi Markandeshwer Deemed Univ, Dept Math, Ambala 133001, Haryana, India

[5] CCS Haryana Agr Univ, Dept Plant Pathol, Hisar 125004, Haryana, India

[6] Univ Cape Town, Dept Pathol, ZA-7700 Cape Town, South Africa

[7] Univ Cape Town, Grad Sch Business, Ports Wood Rd, ZA-8001 Cape Town, South Africa

来源：

ANNALS OF PHYTOMEDICINE-AN INTERNATIONAL JOURNAL | 2024年 / 13卷 / 01期

基金：

英国医学研究理事会;

关键词：

Antioxidants; Secondary metabolites; Neuronal cell lines; OXIDATIVE STRESS; PHYTOCHEMICAL ANALYSIS; PROXIMATE COMPOSITION; PROTEINS GP120; TAT; COUMARIN; CURCUMIN; DAMAGE;

D O I：

10.54085/ap.2024.13.1.90

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

In order to control the neurological complications associated with HIV, it is pertinent to consider the development of an effective antioxidant therapy. The current study was targeted to evaluate the antioxidant capacities of secondary metabolites isolated from Himalayan high-altitude medicinal plants using a standard oxygen radical absorbance capacity assay based on fluorescein (ORAC-Fl assay). Secondary metabolites (SMs) 1-6 were found to be effective in reducing gp120-41 and Tat-generated oxidative stress in SK-N-SH cell lines, quantitated using a fluorescent probe, 5(6)-carboxy-2,7-dichlorofluorescein diacetate (CDFH-DA). These encouraging in vitro preliminary findings suggest that the use of these secondary metabolites may result in the creation of an effective antioxidant therapy that could be used to prevent, delay, or even treat neurological complications linked to oxidative stress caused by HIV proteins.

引用

页码：850 / 855

页数：6