Lactobacillus plantarum-Derived Extracellular Vesicles Modulate Macrophage Polarization and Gut Homeostasis for Alleviating Ulcerative Colitis

被引：15

作者：

Chen, Qian ^{[1
]}

Fang, Zhengzou ^{[2
]}

Yang, Zhe ^{[2
]}

Xv, Xiao ^{[1
]}

Yang, Mengting ^{[1
]}

Hou, Hanjin ^{[1
]}

Li, Zhangzuo ^{[1
]}

Chen, Yanyan ^{[1
,2
,3
]}

Gong, Aihua ^{[1
,2
]}

机构：

[1] Jiangsu Univ, Sch Med, Zhenjiang 212003, Jiangsu, Peoples R China

[2] Jiangsu Univ, Affiliated Hosp, Hematol Dis Inst, Zhenjiang 212003, Peoples R China

[3] Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Taipa 999078, Macao, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2024年 / 72卷 / 26期

关键词：

Lactobacillus plantarum; extracellular vesicles; ulcerative colitis; gut microbiota; macrophages; METABOLISM;

D O I：

10.1021/acs.jafc.4c01758

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Extracellular vesicles released by probiotics have been demonstrated to effectively alleviate intestinal inflammation, yet the precise underlying mechanisms remain unclear. In this research, for the first time, Lactobacillus plantarum UJS001 (LP-UJS) was isolated from fermented sauerkraut in Zhenjiang, China. Thereafter, the therapeutic effect of LP-UJS-derived extracellular vesicles (LP-UJS-EVs) on dextran sulfate sodium-induced ulcerative colitis (UC) in mice was analyzed to elucidate the immune mechanisms. According to our findings, LP-UJS-EVs played a pivotal role in restoring the intestinal barrier and alleviating intestinal inflammation. Notably, LP-UJS-EVs induced M2 polarization of macrophages, promoted the release of IL-10 and TGF-beta, inhibited the release of histamine, IL-6, and TNF-alpha, and exerted regulatory effects on intestinal microflora, as evidenced by the reduced abundances of Coprococcus, Parabacteroides, Staphylococcus, and Allobaculum, alongside the enhanced abundance of Prevotella. Furthermore, both LP-UJS and LP-UJS-EVs affected the lysine degradation pathway and significantly increased the abundance of related metabolites, especially oxoadipic acid. In summary, our results underscore the substantial therapeutic potential of LP-UJS and its secreted EVs in the treatment of UC.

引用

页码：14713 / 14726

页数：14

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