Small RNA and Freeze Survival: The Cryoprotective Functions of MicroRNA in the Frozen Muscle Tissue of the Grey Tree Frog

被引:1
作者
Rehman, Saif [1 ]
Storey, Kenneth B. [1 ]
机构
[1] Carleton Univ, Dept Biol, Ottawa, ON K1S 5B6, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
miRNA; Dryophytes versicolor; metabolic rate depression; epigenetics; cell signalling; gene ontology; METABOLIC-RATE DEPRESSION; NOD-LIKE RECEPTORS; CELL-CYCLE ARREST; PROTEIN-SYNTHESIS; TOLERANCE; PATHWAY; PHOSPHORYLATION; TRANSCRIPTION; ADAPTATION; BIOLOGY;
D O I
10.3390/metabo14070387
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The grey tree frog, Dryophytes versicolor, survives whole-body freezing for weeks during cold winter months. Survival in a state devoid of available food, water, or oxygen forces a reliance on metabolic rate depression (MRD) and the reprioritization of bodily functions. This study utilizes next-generation sequencing (NGS) and bioinformatic analyses to characterize changes in the microRNAome of D. versicolor. When comparing control to frozen groups, five microRNAs (miRNA) were found to be differentially regulated (miR-143-3p, miR-30e-3p, miR-10a-5p, miR-140-3p, and miR-148a-3p), suggesting that they play key roles in freeze survival. The KEGG and GO analyses of these changes predicted a significant negative enrichment of terms associated with cell proliferation and active metabolism while simultaneously predicting the upregulation of cell signalling terms. These results suggest a fast-acting regulatory role for miRNA in contributing to the reorganization of gene expression and the limitation of energy-expensive processes during MRD in the hind leg skeletal muscle of the frog.
引用
收藏
页数:12
相关论文
共 49 条
[1]   Metabolic reorganization in winter: Regulation of pyruvate dehydrogenase (PDH) during long-term freezing and anoxia [J].
Al-attar, Rasha ;
Wijenayake, Sanoji ;
Storey, Kenneth B. .
CRYOBIOLOGY, 2019, 86 :10-18
[2]  
Ardekani Ali M., 2010, Avicenna Journal of Medical Biotechnology, V2, P161
[3]   Cryopreservation An emerging paradigm change [J].
Baust, John G. ;
Gao, Dayong ;
Baust, John M. .
ORGANOGENESIS, 2009, 5 (03) :90-96
[4]   Evidence for cell cycle suppression and microRNA regulation of cyclin D1 during anoxia exposure in turtles [J].
Biggar, Kyle K. ;
Storey, Kenneth B. .
CELL CYCLE, 2012, 11 (09) :1705-1713
[5]   The emerging roles of microRNAs in the molecular responses of metabolic rate depression [J].
Biggar, Kyle K. ;
Storey, Kenneth B. .
JOURNAL OF MOLECULAR CELL BIOLOGY, 2011, 3 (03) :167-175
[6]   TO FREEZE OR NOT TO FREEZE - INVERTEBRATE SURVIVAL OF SUBZERO TEMPERATURES [J].
BLOCK, W .
FUNCTIONAL ECOLOGY, 1991, 5 (02) :284-290
[7]   Poly(A)-binding protein is differentially required for translation mediated by viral internal ribosome entry sites [J].
Bradrick, Shelton S. ;
Dobrikova, Elena Y. ;
Kaiser, Constanze ;
Shveygert, Mayya ;
Gromeier, Matthias .
RNA, 2007, 13 (09) :1582-1593
[8]  
Breedon SA, 2022, OXYGEN-BASEL, V2, P227, DOI 10.3390/oxygen2020017
[9]   Signaling Kinase AMPK Activates Stress-Promoted Transcription via Histone H2B Phosphorylation [J].
Bungard, David ;
Fuerth, Benjamin J. ;
Zeng, Ping-Yao ;
Faubert, Brandon ;
Maas, Nancy L. ;
Viollet, Benoit ;
Carling, David ;
Thompson, Craig B. ;
Jones, Russell G. ;
Berger, Shelley L. .
SCIENCE, 2010, 329 (5996) :1201-1205
[10]   MicroRNA functions [J].
Bushati, Natascha ;
Cohen, Stephen M. .
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 2007, 23 :175-205