Phosvitin phosphopeptides alleviate the inhibitory effect of oxidative stress on osteoblast function through FoxO/Wnt signaling

Oxidative stress inhibits osteoblast differentiation and function, leading to the development of osteoporosis. The effects of Phosvitin phosphopeptides (PPPs) on the osteogenic function of osteoblast were determined based on oxidative stress MC3T3-E1 cell model induced by hydrogen peroxide. The results showed that 50 mu g/mL of PPPs significantly increased cell viability from 56.00% (H 2 O 2 ) to 106.80% for 72 h. DCFH-DA fluorescence assay demonstrated that PPPs could remarkably reduce reactive oxygen species levels induced by H 2 O 2 . The alkaline phosphatase activity of oxidative stress -treated osteoblast increased from 2.36 Kim ' unit/100 mL (H 2 O 2 group) to 8.16 Kim ' unit/100 mL, along with an increase in the activity of total superoxide dismutase, glutathione peroxidase, catalase, and the number of mineralized nodules in response to PPPs treatment. Overall, the results of this work indicated that PPPs had a stimulating impact on the Wnt/ 13 -catenin signaling pathway while simultaneously inhibiting the forkhead box class O ( FoxO) signaling pathway in MC3T3-E1 osteoblast induced by H 2 O 2 . Consequently, these findings demonstrate that PPPs have the ability to protect the osteogenic function of MC3T3-E1 osteoblasts against oxidative damage through the activation of the FoxO/Wnt signaling pathway.