Rapid Plasma Proteome Profiling via Nanoparticle Protein Corona and Direct Infusion Mass Spectrometry

被引:0
作者
Jiang, Yuming [1 ,2 ]
Meyer, Jesse G. [1 ,2 ]
机构
[1] Cedars Sinai Med Ctr, Adv Clin Biosyst Res Inst, Dept Computat Biomed, Los Angeles, CA 90048 USA
[2] Cedars Sinai Med Ctr, Smidt Heart Inst, Los Angeles, CA 90048 USA
关键词
plasma proteomics; high throughput; directinfusion; ion mobility; nanoparticles; DISPA; proteomics; peptides;
D O I
10.1021/acs.jproteome.4c00302
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Noninvasive detection of protein biomarkers in plasma is crucial for clinical purposes. Liquid chromatography-mass spectrometry (LC-MS) is the gold standard technique for plasma proteome analysis, but despite recent advances, it remains limited by throughput, cost, and coverage. Here, we introduce a new hybrid method that integrates direct infusion shotgun proteome analysis (DISPA) with nanoparticle (NP) protein corona enrichment for high-throughput and efficient plasma proteomic profiling. We realized over 280 protein identifications in 1.4 min collection time, which enables a potential throughput of approximately 1000 samples daily. The identified proteins are involved in valuable pathways, and 44 of the proteins are FDA-approved biomarkers. The robustness and quantitative accuracy of this method were evaluated across multiple NPs and concentrations with a mean coefficient of variation of 17%. Moreover, different protein corona profiles were observed among various NPs based on their distinct surface modifications, and all NP protein profiles exhibited deeper coverage and better quantification than neat plasma. Our streamlined workflow merges coverage and throughput with precise quantification, leveraging both DISPA and NP protein corona enrichment. This underscores the significant potential of DISPA when paired with NP sample preparation techniques for plasma proteome studies.
引用
收藏
页码:3649 / 3658
页数:10
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