A 14-amino acid cationic peptide Bolespleenin334-347 from the marine fish mudskipper Boleophthalmus pectinirostris exhibiting potent antimicrobial activity and therapeutic potential

被引:4
作者
Bai, Yuqi [1 ]
Zhang, Weibin [1 ]
Zheng, Wenbin [1 ]
Meng, Xin-Zhan [1 ]
Duan, Yingyi [1 ]
Zhang, Chang [1 ]
Chen, Fangyi [1 ,2 ,3 ]
Wang, Ke-Jian [1 ,2 ,3 ]
机构
[1] Xiamen Univ, Coll Ocean & Earth Sci, State Key Lab Marine Environm Sci, Xiamen, Fujian, Peoples R China
[2] Xiamen Univ, Coll Ocean & Earth Sci, State Prov Joint Engn Lab Marine Bioprod & Technol, Xiamen, Fujian, Peoples R China
[3] Xiamen Univ, Fujian Innovat Res Inst Marine Biol Antimicrobial, Coll Ocean & Earth Sci, Xiamen, Peoples R China
基金
中国国家自然科学基金;
关键词
Mudskipper antimicrobial peptide; Bolespleenin; 334-347; Membrane disruption; Superficial skin infection; MRSA; RESISTANT STAPHYLOCOCCUS-AUREUS; SKIN; EPIDEMIOLOGY; INFECTIONS; ANTIBIOTICS; ARGININE; IMPACT; LYSINE; AGENTS; MICE;
D O I
10.1016/j.bcp.2024.116344
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Antimicrobial peptides (AMPs) are an important component of innate immunity in both vertebrates and invertebrates, and some of the unique characteristics of AMPs are usually associated with their living environment. The marine fish, mudskipper Boleophthalmus pectinirostris, usually live amphibiously in intertidal environments that are quite different from other fish species, which would be an exceptional source of new AMPs. In the study, an AMP named Bolespleenin334-347 was identified, which was a truncated peptide derived from a new functional gene found in B. pectinirostris, that was up-regulated in response to bacterial challenge. Bolespleenin334-347 had only 14 amino acid residues, including five consecutive arginine residues. It was found that the peptide had broad-spectrum antibacterial activity, good thermal stability and sodium ion tolerance. Bolespleenin334-347 killed Acinetobacter baumannii and Staphylococcus aureus by disrupting the structural integrity of the bacterial membrane, leading to leakage of the cellular contents, and inducing accumulation of bacterial endogenous reactive oxygen species (ROS). In addition, Bolespleenin334-347 effectively inhibited biofilm formation of A. baumannii and S. aureus and long-term treatment did not lead to the development of resistance. Importantly, Bolespleenin334-347 maintained stable activity against clinically multi-drug resistant bacterial strains. In addition, it was noteworthy that Bolespleenin334-347 showed superior efficacy to LL-37 and vancomycin in a constructed mouse model of MRSA-induced superficial skin infections, as evidenced by a significant reduction in bacterial load and more favorable wound healing. This study provides an effective antimicrobial agent for topical skin infections with potential therapeutic efficacy for infections with drug-resistant bacteria, including MRSA.
引用
收藏
页数:16
相关论文
共 83 条
  • [41] Topical versus Systemic Antimicrobial Therapy for Treating Mildly Infected Diabetic Foot Ulcers: A Randomized, Controlled, Double-Blinded, Multicenter Trial of Pexiganan Cream
    Lipsky, Benjamin A.
    Holroyd, Kenneth J.
    Zasloff, Michael
    [J]. CLINICAL INFECTIOUS DISEASES, 2008, 47 (12) : 1537 - 1545
  • [42] The Synergistic Effect of Mud Crab Antimicrobial Peptides Sphistin and Sph12-38 With Antibiotics Azithromycin and Rifampicin Enhances Bactericidal Activity Against Pseudomonas Aeruginosa
    Liu, Jie
    Chen, Fangyi
    Wang, Xiaofei
    Peng, Hui
    Zhang, Hua
    Wang, Ke-Jian
    [J]. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, 2020, 10
  • [43] Antimicrobial Peptide Cec4 Eradicates the Bacteria of Clinical Carbapenem-ResistantAcinetobacter baumanniiBiofilm
    Liu, Weiwei
    Wu, Zhaoying
    Mao, Chengju
    Guo, Guo
    Zeng, Zhu
    Fei, Ying
    Wan, Shan
    Peng, Jian
    Wu, Jianwei
    [J]. FRONTIERS IN MICROBIOLOGY, 2020, 11
  • [44] Efficacy of topical and systemic antibiotic treatment of meticillin-resistant Staphylococcus aureus in a murine superficial skin wound infection model
    Lundberg, Carina Vingsbo
    Frimodt-Moller, Niels
    [J]. INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2013, 42 (03) : 272 - 275
  • [45] Antimicrobial Peptides : An Emerging Category of Therapeutic Agents
    Mahlapuu, Margit
    Hakansson, Joakim
    Ringstad, Lovisa
    Bjorn, Camilla
    [J]. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, 2016, 6
  • [46] Gram-positive bacterial cell envelopes: The impact on the activity of antimicrobial peptides
    Malanovic, Nermina
    Lohner, Karl
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2016, 1858 (05): : 936 - 946
  • [47] In vitro and in vivo antibiofilm activity of the synthetic antimicrobial peptide WLBU2 against multiple drug resistant Pseudomonas aeruginosa strains
    Masihzadeh, Sara
    Amin, Mansour
    Farshadzadeh, Zahra
    [J]. BMC MICROBIOLOGY, 2023, 23 (01)
  • [48] OPINION Antimicrobial peptides: linking partition, activity and high membrane-bound concentrations
    Melo, Manuel N.
    Ferre, Rafael
    Castanho, Miguel A. R. B.
    [J]. NATURE REVIEWS MICROBIOLOGY, 2009, 7 (03) : 245 - 250
  • [49] Arginine- and Lysine-rich Peptides: Synthesis, Characterization and Antimicrobial Activity
    Mollica, Adriano
    Macedonio, Giorgia
    Stefanucci, Azzurra
    Costante, Roberto
    Carradori, Simone
    Cataldi, Valentina
    Di Giulio, Mara
    Cellini, Luigina
    Silvestri, Romano
    Giordano, Cesare
    Scipioni, Anita
    Morosetti, Stefano
    Punzi, Pasqualina
    Mirzaie, Sako
    [J]. LETTERS IN DRUG DESIGN & DISCOVERY, 2018, 15 (03) : 220 - 226
  • [50] Mor A, 2000, DRUG DEVELOP RES, V50, P440, DOI 10.1002/1098-2299(200007/08)50:3/4<440::AID-DDR27>3.3.CO