Molecular Epidemiology and Phylogeny of Theileria ovis and Theileria lestoquardi in Sheep and Goats from Southern Punjab, Pakistan

被引：0

作者：

Riaz, Muhammad ^{[1
]}

Chang, Shun-Chung ^{[2
]}

Tasawar, Zahida ^{[1
]}

Sajid, Muhammad ^{[1
]}

Nasreen, Nasreen ^{[3
]}

Amaro-Estrada, Itzel ^{[4
]}

Cossio-Bayugar, Raquel ^{[5
]}

de la Fuente, Jose ^{[6
,7
]}

Alanazi, Abdullah D. ^{[8
]}

Chen, Chien-Chin ^{[9
,10
,11
,12
]}

Khan, Adil ^{[3
,13
,14
]}

Ben Said, Mourad ^{[15
,16
]}

机构：

[1] Bahauddin Zakariya Univ, Inst Pure & Appl Biol, Zool Div, Multan, Pakistan

[2] Chia Yi Christian Hosp, Ditmanson Med Fdn, Dept Emergency Med, Chiayi, Taiwan

[3] Bacha Khan Univ, Dept Bot & Zool, Charsadda, Khyber Pakhtunk, Pakistan

[4] INIFAP, Ctr Nacl Invest Disciplinaria Salud Anim & Inocu, INIFAP, Carretera Fed Cuernavaca Cuautla 8534,Col Progreso, Jiutepec, Morelos, Mexico

[5] Inst Nacl Invest Forestales Agr & Pecuarias INIFAP, Ctr Nacl Invest Disciplinarias Salud Anim & Inocu, Dept Artropodol, Blvd Cuauhnahuac 8534, Jiutepec, Morelos, Mexico

[6] UCLM, Inst Invest Recursos Cineget IREC, CSIC, JCCM,SaBio, Ronda Toledo 12, Ciudad Real 13071, Spain

[7] Oklahoma State Univ, Ctr Vet Hlth Sci, Dept Vet Pathobiol, Stillwater, OK 74078 USA

[8] Shaqra Univ, Fac Sci & Humanities, Dept Biol Sci, Ad Dawadimi 1040, Saudi Arabia

[9] Chia Yi Christian Hosp, Ditmanson Med Fdn, Dept Pathol, Chiayi, Taiwan

[10] Chia Nan Univ Pharm & Sci, Dept Cosmet Sci, Tainan, Taiwan

[11] Natl Chung Hsing Univ, Rong Hsing Res Ctr Translat Med, PhD Program Translat Med, Taichung, Taiwan

[12] Natl Cheng Kung Univ, Coll Biosci & Biotechnol, Dept Biotechnol & Bioind Sci, Tainan, Taiwan

[13] Bacha Khan Univ, Dept Bot Zool, Charsadda, Khyber Pakhtunk, Pakistan

[14] Mt Allison Univ, Dept Biol, Sackville, NB, Canada

[15] Univ Manouba, Natl Sch Vet Med Sidi Thabet, Lab Microbiol, Manouba 2010, Tunisia

[16] Univ Manouba, Higher Inst Biotechnol Sidi Thabet, Dept Basic Sci, Manouba, Tunisia

来源：

VECTOR-BORNE AND ZOONOTIC DISEASES | 2024年 / 24卷 / 10期

关键词：

Theileria ovis; Theileria lestoquardi; small ruminants; molecular detection; phylogenetic analysis; Pakistan; POLYMERASE-CHAIN-REACTION; SMALL RUMINANTS; PCR-RFLP; GENETIC DIVERSITY; BABESIA-OVIS; IDENTIFICATION; PREVALENCE; TICKS; INFECTION; DISTRICTS;

D O I：

10.1089/vbz.2023.0118

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Background: Theileria spp. are responsible for ovine and caprine theileriosis, leading to significant morbidity and mortality in small ruminants. The present study aims to investigate Theileria spp. infections in small ruminants from Southern Punjab in Pakistan, and genetic characterize revealed Theileria spp. isolates. Methods: A total of 93 sheep and 107 goats were sampled between May and August 2022. Blood smears were examined microscopically, and PCR amplification targeting the 18S rRNA gene was performed to detect Theileria spp. Additionally, specific PCR assays targeting 18S rRNA and ms1 partial sequences were used to identify Theileria ovis and T. lestoquardi, respectively. Results: The prevalence of Theileria spp. was significantly higher using PCR (13.5%) compared to microscopic screening (5%). Sheep showed a higher prevalence rate (19.4%) compared to goats (8.4%) (p = 0.024). Young sheep aged <= 1 year were more commonly infected with Theileria spp. (41%) compared to older sheep (p = 0.006). The prevalence of Theileria spp. was higher in sheep-only herds (37.3%) compared to goat-only herds (18%) or mixed-species herds (8.1%) (p = 0.015). The prevalence rates of T. ovis and T. lestoquardi were 9% and 2.5%, respectively, with four animals (2 goats and 2 sheep) showing co-infection. Phylogenetic analysis revealed that our T. ovis 18S rRNA sequence clustered with previously reported sequences from sheep in Turkey, China, Spain, and goats in Tanzania. The obtained T. lestoquardi ms1 partial sequence formed a distinct cluster from other T. lestoquardi isolates in Pakistan and neighboring countries. Conclusion: Theileria spp. co-circulation in Pakistani small ruminants, particularly the presence of T. ovis and T. lestoquardi, highlights the need for attention from animal health decision-makers due to their financial and health impacts.

引用

页码：656 / 665

页数：10

共 60 条

[1] Molecular detection and prevalence of Theileria ovis and Anaplasma marginale in sheep blood samples collected from Layyah district in Punjab, Pakistan [J].