Ginger Polyphenols Reverse Molecular Signature of Amygdala Neuroimmune Signaling and Modulate Microbiome in Male Rats with Neuropathic Pain: Evidence for Microbiota-Gut-Brain Axis

被引:2
作者
Shen, Chwan-Li [1 ,2 ,3 ]
Santos, Julianna Maria [1 ]
Elmassry, Moamen M. [4 ]
Bhakta, Viren [5 ]
Driver, Zarek [6 ]
Ji, Guangchen [7 ]
Yakhnitsa, Vadim [7 ]
Kiritoshi, Takaki [7 ]
Lovett, Jacob [1 ]
Hamood, Abdul Naji [8 ]
Sang, Shengmin [9 ]
Neugebauer, Volker [2 ,3 ,7 ]
机构
[1] Texas Tech Univ, Hlth Sci Ctr, Dept Pathol, Lubbock, TX 79430 USA
[2] Texas Tech Univ, Hlth Sci Ctr, Ctr Excellence Integrat Hlth, Lubbock, TX 79430 USA
[3] Texas Tech Univ, Hlth Sci Ctr, Ctr Excellence Translat Neurosci & Therapeut, Lubbock, TX 79430 USA
[4] Princeton Univ, Dept Mol Biol, Princeton, NJ 08540 USA
[5] Texas Tech Univ, Dept Biol, Lubbock, TX 79401 USA
[6] Texas Tech Univ, Dept Biochem, Lubbock, TX 79401 USA
[7] Texas Tech Univ, Hlth Sci Ctr, Dept Pharmacol & Neurosci, Lubbock, TX 79430 USA
[8] Texas Tech Univ, Hlth Sci Ctr, Dept Microbiol & Infect Dis, Lubbock, TX 79430 USA
[9] North Carolina A&T State Univ, Lab Funct Foods & Human Hlth, Ctr Excellence Post Harvest Technol, North Carolina Res Campus, Kannapolis, NC 28081 USA
关键词
ginger; pain; gut microbiome; neuroinflammation; brain; HISTONE DEACETYLASE 2; GADD45B-DEFICIENT MICE; HEMATOPOIETIC-CELLS; GENE-EXPRESSION; DORSAL-HORN; STRESS; MODEL; GADD45A-DEFICIENT; HIPPOCAMPUS; SUPPRESSION;
D O I
10.3390/antiox13050502
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Emerging evidence shows that the gut microbiota plays an important role in neuropathic pain (NP) via the gut-brain axis. Male rats were divided into sham, spinal nerve ligation (SNL), SNL + 200 mg GEG/kg BW (GEG200), and SNL + 600 mg GEG/kg BW (GEG600) for 5 weeks. The dosages of 200 and 600 mg GEG/kg BW for rats correspond to 45 g and 135 g raw ginger for human daily consumption, respectively. Both GEG groups mitigated SNL-induced NP behavior. GEG-supplemented animals had a decreased abundance of Rikenella, Muribaculaceae, Clostridia UCG-014, Mucispirillum schaedleri, RF39, Acetatifactor, and Clostridia UCG-009, while they had an increased abundance of Flavonifactor, Hungatella, Anaerofustis stercorihominis, and Clostridium innocuum group. Relative to sham rats, Fos and Gadd45g genes were upregulated, while Igf1, Ccl2, Hadc2, Rtn4rl1, Nfkb2, Gpr84, Pik3cg, and Abcc8 genes were downregulated in SNL rats. Compared to the SNL group, the GEG200 group and GEG600 group had increases/decreases in 16 (10/6) genes and 11 (1/10) genes, respectively. GEG downregulated Fos and Gadd45g genes and upregulated Hdac2 genes in the amygdala. In summary, GEG alleviates NP by modulating the gut microbiome and reversing a molecular neuroimmune signature.
引用
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页数:17
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