Isorhamnetin Improves Oocyte Maturation by Activating the Pi3k/Akt Signaling Pathway

ScopeIsorhamnetin is a natural flavonoid with various pharmacological activities, which can be widely and continuously ingested by humans and animals through their daily diet. The aim of this study is to explore the benefits and molecular mechanisms of isorhamnetin on oocyte maturation.Methods and resultsOocytes are incubated with isorhamnetin (5, 10, 20, and 30 mu M) for 44 h. Isorhamnetin (10 mu M) increases the polar body extrusion rate of oocytes. Furthermore, isorhamnetin alleviates oxidative stress by inhibiting reactive oxygen species levels and stimulating SOD2 protein expression. The changes in intracellular mitochondrial autophagy and apoptosis-related proteins (Bcl-2, Bax/Bcl-2, and C-Casp3) indicate that isorhamnetin inhibits oocyte apoptosis. Isorhamnetin inhibits endoplasmic reticulum stress by reducing the protein expression of CHOP and GRP78 and improving the normal distribution rate of endoplasmic reticulum. Mechanistic studies show that isorhamnetin activates the PI3K/Akt signaling pathway.ConclusionIsorhamnetin promotes oocyte maturation by inhibiting oxidative stress, mitochondrial dysregulation, apoptosis, and endoplasmic reticulum stress, which have important potential for improving oocyte quality and treating female infertility. A possible mechanism by which isorhamnetin promotes porcine oocyte maturation. Isorhamnetin enhances polar body extrusion rate and mitochondrial function, but inhibits reactive oxidative stress, apoptosis, and ER stress to promote oocyte maturation. Delta Psi m, mitochondrial membrane potential. image