Advancing Point-of-Care Applications with Droplet Microfluidics: From Single-Cell to Multicellular Analysis

被引：2
|
作者：
Sharkey, Christina [1 ,2 ]
White, Rachel [1 ]
Finocchiaro, Michael [1 ]
Thomas, Judene [1 ,3 ]
Estevam, Jose [1 ]
Konry, Tania [1 ]
机构：
[1] Northeastern Univ, Dept Pharmaceut Sci, Boston, MA 02115 USA
[2] Harvard Med Sch, Div Urol, Dept Surg, Beth Israel Deaconess Med Ctr, Boston, MA USA
[3] Northeastern Univ, Dept Chem & Chem Biol, Boston, MA USA
来源：
ANNUAL REVIEW OF BIOMEDICAL ENGINEERING | 2024年 / 26卷
关键词：
droplet microfluidics; multicellular droplet microfluidics; single-cell microfluidics; immuno-oncology; HIGH-THROUGHPUT; EXTRACELLULAR-MATRIX; DRUG-RESISTANCE; TUMOR; CHIP; GENERATION; PLATFORM; MODEL; DEVICES; CULTURE;
D O I：
10.1146/annurev-bioeng-110222-102142
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Recent advances in single-cell and multicellular microfluidics technology have provided powerful tools for studying cancer biology and immunology. The ability to create controlled microenvironments, perform high-throughput screenings, and monitor cellular interactions at the single-cell level has significantly advanced our understanding of tumor biology and immune responses. We discuss cutting-edge multicellular and single-cell microfluidic technologies and methodologies utilized to investigate cancer-immune cell interactions and assess the effectiveness of immunotherapies. We explore the advantages and limitations of the wide range of 3D spheroid and single-cell microfluidic models recently developed, highlighting the various approaches in device generation and applications in immunotherapy screening for potential opportunities for point-of-care approaches.
引用
收藏
页码：119 / 139
页数：21
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