Surface Engineering for Endothelium-Mimicking Functions to Combat Infection and Thrombosis in Extracorporeal Life Support Technologies

被引：5

作者：

Ashcraft, Morgan ^{[1
]}

Garren, Mark ^{[2
]}

Lautner-Csorba, Orsolya ^{[3
]}

Pinon, Vicente ^{[1
]}

Wu, Yi ^{[2
]}

Crowley, Dagney ^{[2
]}

Hill, Joseph ^{[3
]}

Morales, Yeniselis ^{[3
]}

Bartlett, Robert ^{[3
]}

Brisbois, Elizabeth J. ^{[2
]}

Handa, Hitesh ^{[1
,2
]}

机构：

[1] Univ Georgia, Coll Pharm, Dept Pharmaceut & Biomed Sci, Athens, GA 30602 USA

[2] Univ Georgia, Coll Engn, Sch Chem Mat & Biomed Engn, Athens, GA 30602 USA

[3] Univ Michigan, Dept Surg, Med Ctr, Ann Arbor, MI 48109 USA

来源：

ADVANCED HEALTHCARE MATERIALS | 2024年 / 13卷 / 22期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

antifouling; antimicrobial; hemocompatible; nitric oxide; polyethylene glycol; VASODILATOR-STIMULATED PHOSPHOPROTEIN; N-ACETYLPENICILLAMINE SNAP; PEG-MODIFIED POLYURETHANE; OXIDE RELEASING POLYMER; NITRIC-OXIDE; MEMBRANE-OXYGENATION; POLYETHYLENE-GLYCOL; PROTEIN ADSORPTION; HUMAN PLATELETS; RABBIT MODEL;

D O I：

10.1002/adhm.202400492

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Blood-contacting medical devices routinely fail from the cascading effects of biofouling toward infection and thrombosis. Nitric oxide (NO) is an integral part of endothelial homeostasis, maintaining platelet quiescence and facilitating oxidative/nitrosative stress against pathogens. Recently, it is shown that the surface evolution of NO can mediate cell-surface interactions. However, this technique alone cannot prevent the biofouling inherent in device failure with dynamic blood-contacting applications. This work proposes an endothelium-mimicking surface design pairing controlled NO release with an inherently antifouling polyethylene glycol interface (NO+PEG). This simple, robust, and scalable platform develops surface-localized NO availability with surface hydration, leading to a significant reduction in protein adsorption as well as bacteria/platelet adhesion. Further in vivo thrombogenicity studies show a decrease in thrombus formation on NO+PEG interfaces, with preservation of circulating platelet and white blood cell counts, maintenance of activated clotting time, and reduced coagulation cascade activation. It is anticipated that this bio-inspired surface design will enable a facile alternative to existing surface technologies to address clinical manifestations of infection and thrombosis in dynamic blood-contacting environments. Vascular devices routinely fail due to surface-induced thrombosis and infection. Nitric oxide (NO) is a promising agent to prevent platelet activation and microbial growth but cannot prevent any underlying protein surface fouling. A hierarchical strategy of controlled NO release with hydrophilic polyethylene glycol polymer interfaces is described for improved hemocompatibility and device patency in dynamic blood-contacting applications. image

引用

页数：16