MsMIOX2, encoding a MsbZIP53-activated myo-inositol oxygenase, enhances saline-alkali stress tolerance by regulating cell wall pectin and hemicellulose biosynthesis in alfalfa

被引：1

作者：

Guo, Weileng ^{[1
]}

Chen, Jiaxin ^{[1
]}

Liu, Lei ^{[1
]}

Ren, Yuekun ^{[1
]}

Guo, Rui ^{[1
]}

Ding, Yang ^{[1
]}

Li, Ying ^{[1
]}

Chai, Juqi ^{[1
]}

Sun, Yuanqing ^{[1
]}

Guo, Changhong ^{[1
]}

机构：

[1] Harbin Normal Univ, Coll Life Sci & Technol, Key Lab Mol & Cytogenet, Harbin 150025, Peoples R China

来源：

PLANT JOURNAL | 2024年 / 120卷 / 03期

基金：

中国国家自然科学基金; 国家重点研发计划;

关键词：

Medicago sativa L; saline-alkali stress; pectin; hemicellulose; cell wall; MsMIOX2; MsbZIP53; DROUGHT TOLERANCE; GENE; ARABIDOPSIS; IDENTIFICATION; EXPRESSION; GROWTH;

D O I：

10.1111/tpj.17032

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Alfalfa is one of the most widely cultivated forage crops worldwide. However, soil salinization restricts alfalfa growth and development and affects global productivity. The plant cell wall is the first barrier against various stresses. Therefore, elucidating the alterations in cell wall architecture is crucial for stress adaptation. This study aimed to clarify the impact of myo-inositol oxygenase 2 (MsMIOX2) on cell wall pectin and hemicellulose biosynthesis under saline-alkali stress and identify the upstream transcription factors that govern MsMIOX2. MsMIOX2 activation induced cell wall pectin and hemicellulose accumulation under saline-alkali stress. The effects of MsMIOX2 in saline-alkali tolerance were investigated by characterizing its overexpression and RNA interference lines. MsMIOX2 overexpression positively regulated the antioxidant system and photosynthesis in alfalfa under saline-alkali stress. MsMIOX2 exhibited myo-inositol oxygenase activity, which increased polysaccharide contents, facilitated pectin and hemicellulose biosynthesis, and extended the cell wall thickness. However, MsMIOX2 RNA interference decreased cell wall thickness and alleviated alfalfa saline-alkali stress tolerance. In addition, MsbZIP53 was identified as an upstream transcriptional MsMIOX2 regulator by yeast one-hybrid, electrophoretic mobility shift assay, dual-luciferase, and beta-glucuronidase assays. MsbZIP53 overexpression increased MsMIOX2 expression, elevated MIOX activity, reinforced the antioxidant system and photosynthesis, and increased saline-alkali stress tolerance in alfalfa. In conclusion, this study presents a novel perspective for elucidating the molecular mechanisms of saline-alkali stress tolerance in alfalfa and emphasizes the potential use of MsMIOX2 in alfalfa breeding.

引用

页码：998 / 1013

页数：16