Integrating Graphene Oxide-Hydrogels and Electrical Stimulation for Controlled Neurotrophic Factor Encapsulation: A Promising Approach for Efficient Nerve Tissue Regeneration

被引:1
作者
Mendes, Alexandre Xavier [1 ,2 ]
Aguilar, Lilith Caballero [2 ,3 ,4 ]
do Nascimento, Adriana Teixeira [1 ,2 ]
Duchi, Serena [2 ,5 ]
Charnley, Mirren [6 ,7 ,8 ]
Nisbet, David R. [2 ,3 ,4 ,9 ]
Quigley, Anita F. [1 ,10 ,11 ]
Kapsa, Robert M. I. [1 ,2 ,10 ,11 ]
Silva, Saimon Moraes [13 ]
Moulton, Simon E. [1 ,2 ,12 ]
机构
[1] Swinburne Univ Technol, ARC Ctr Excellence Electromat Sci, Sch Sci Comp & Engn Technol, Melbourne, Vic 3122, Australia
[2] St Vincents Hosp Melbourne, Aikenhead Ctr Med Discovery, Melbourne, Vic 3065, Australia
[3] Univ Melbourne, Graeme Clark Inst, Biomed Engn Dept, Melbourne, Vic 3065, Australia
[4] Univ Melbourne, Fac Engn & Informat Technol, Dept Biomed Engn, Melbourne, Vic 3010, Australia
[5] Univ Melbourne, St Vincents Hosp Melbourne, Dept Surg, Melbourne, Vic 3065, Australia
[6] Swinburne Univ Technol, Ctr Opt Sci, Dept Hlth Sci & Biostat, Hawthorn, Vic 3122, Australia
[7] Swinburne Univ Technol, Dept Hlth Sci & Biostat, Hawthorn, Vic 3122, Australia
[8] Peter MacCallum Canc Ctr, Immune Signalling Lab, Melbourne, Vic 8006, Australia
[9] Univ Melbourne, Fac Med Dent & Hlth Sci, Melbourne Med Sch, Melbourne, Vic 3010, Australia
[10] RMIT Univ, Sch Elect & Biomed Engn, Melbourne, Vic 3001, Australia
[11] Univ Melbourne, Dept Med, St Vincents Hosp Melbourne, Melbourne, Vic 3065, Australia
[12] Swinburne Univ Technol, Iverson Hlth Innovat Res Inst, Melbourne, Vic 3122, Australia
[13] La Trobe Univ, La Trobe Inst Mol Sci, Biomed & Environm Sensor Technol Ctr, Dept Biochem & Chem, Melbourne, Vic 3086, Australia
来源
ACS APPLIED BIO MATERIALS | 2024年 / 7卷 / 06期
基金
澳大利亚研究理事会;
关键词
NGF release; NGF uptake; nerve growth factor; NGF; GelMA; GelMA/GO; electroactivehydrogels; electrical stimulation; PC12; CELL; GROWTH-FACTOR; NEURITE OUTGROWTH; BETA-TUBULIN; PC-12; CELLS; DIFFERENTIATION; EXPRESSION; SCAFFOLDS; NANOFIBER; DELIVERY;
D O I
10.1021/acsabm.4c00523
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
Nerve growth factor (NGF) plays a crucial role in cellular growth and neurodifferentiation. To achieve significant neuronal regeneration and repair using in vitro NGF delivery, spatiotemporal control that follows the natural neuronal processes must be developed. Notably, a challenge hindering this is the uncontrolled burst release from the growth factor delivery systems. The rapid depletion of NGF reduces treatment efficacy, leading to poor cellular response. To address this, we developed a highly controllable system using graphene oxygen (GO) and GelMA hydrogels modulated by electrical stimulation. Our system showed superior control over the release kinetics, reducing the burst up 30-fold. We demonstrate that the system is also able to sequester and retain NGF up to 10-times more efficiently than GelMA hydrogels alone. Our controlled release system enabled neurodifferentiation, as revealed by gene expression and immunostaining analysis. The increased retention and reduced burst release from our system show a promising pathway for nerve tissue engineering research toward effective regeneration.
引用
收藏
页码:4175 / 4192
页数:18
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