Microplastics and nanoplastics in environment: Sampling, characterization and analytical methods

Microplastics (MPs) and nanoplastics (NPs) have gained increasing attention in environmental research due to their ubiquitousness and potential impacts on natural environments and human health as per the UN Sustainable Development Goals (SDGs), particularly SDG-14 to address global threats where at least 12 SDGs, directly/ indirectly impacts. Present review is undertaken to highlight the process of breakdown of diverse groups of plastic products in soil, surface water, and groundwater under the influence of different factors (UV, light, heat, microbe, etc.), which are mobilized as MPs/NPs to the surface water, groundwater, air, soil, and living organisms by different natural and anthropogenic processes. Review also highlights a comprehensive overview of the methodology for sampling, characterization, and analysis for these minuscule plastic particles (PPs) in various environmental samples, encompassing surface/subsurface water, sediments, soils, and biological organisms. The collection, extraction, and characterization of MPs/NPs, typically employ filtration processes, wherein a known volume of water is passed through a fine mesh to capture MPs/NPs from water samples. Sediment/soil samples require sieving and density separation techniques to isolate PPs from the surrounding matrix. Biological samples require digestion steps to remove organic matter, leaving behind plastics for analysis. Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy, and scanning electron microscopy (SEM) are commonly utilized to determine the polymer composition, size, shape, and surface characteristics of PPs. Quantification involves several approaches, visual counting, image analysis, and spectroscopic techniques. Abundance of MPs/NPs in the respective environmental samples (water, air, soil, etc.) can be determined by comparing the richness (i.e., number/mass) of plastics to the volume or weight of the original sample. Such comprehensive analytical methodologies contribute to understand the scope and magnitude of plastic pollution and its potential repercussions for ecosystems and human well-being, which are essential for developing the effective strategies to mitigate these pressing global environmental challenges for sustainable development.