Essential Role of Methyl Groups in Ice Recrystallization Inhibition of Antifreeze Silver Nanoparticles Studied by Surface-Enhanced Raman Scattering

被引:1
作者
Fukunaga, Yu [1 ]
Okada, Tetsuo [1 ,2 ]
机构
[1] Tokyo Inst Technol, Dept Chem, Tokyo 1528551, Japan
[2] Natl Inst Technol KOSEN, Numazu Coll, Numazu, Shizuoka 4108501, Japan
基金
日本学术振兴会;
关键词
BINDING PROTEINS; HYDROGEN-BONDS; WATER; ADSORPTION; HYDRATE; 2-MERCAPTOPYRIMIDINE; RECOGNITION; TOXICITY; GROWTH;
D O I
10.1021/acs.jpcc.4c00998
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Controlling ice recrystallization is of fundamental importance in a variety of applications, including the preservation of food, cells, and tissue samples used in regenerative medicine, to prevent damage from freezing. Despite the high demand for ice recrystallization inhibition (IRI) materials, their rational design remains challenging due to a lack of understanding of the IRI mechanism at the molecular level. In this study, the antifreeze behavior of silver nanoparticles (AgNPs) modified with mercaptopyrimidine derivatives is studied using surface-enhanced Raman scattering (SERS). AgNPs exhibit IRI activity when modified with molecules that have outward-facing methyl groups. In contrast, pyrimidine derivative monomers do not exhibit IRI activity, suggesting that immobilization of the molecules on the AgNP surface is important. SERS spectra indicate that the interaction between outward-facing methyl groups and liquid water molecules is enhanced upon freezing. No direct binding between methyl groups and ice is confirmed. Thus, the IRI activity of antifreeze AgNPs can be reasonably understood by assuming the formation of a clathrate-like hydration shell in the vicinity of the methyl groups. Liquid water is stabilized by hydrophobic hydration, resulting in insufficient water available for ice crystal growth and higher IRI activity. This work provides fundamental insights into the design of IRI materials that have high potential in cell cryopreservation and other IRI applications.
引用
收藏
页码:8068 / 8076
页数:9
相关论文
共 65 条
[11]   Molecular Insight into the Adsorption of Spruce Budworm Antifreeze Protein to an Ice Surface: A Clathrate-Mediated Recognition Mechanism [J].
Chakraborty, Sandipan ;
Jana, Biman .
LANGMUIR, 2017, 33 (28) :7202-7214
[12]   A diminished role for hydrogen bonds in antifreeze protein binding to ice [J].
Chao, HM ;
Houston, ME ;
Hodges, RS ;
Kay, CM ;
Sykes, BD ;
Loewen, MC ;
Davies, PL ;
Sonnichsen, FD .
BIOCHEMISTRY, 1997, 36 (48) :14652-14660
[13]  
Cohen-Adad R., 1991, SolubilityData Series
[14]   Structure and dynamics of nanoconfined water and aqueous solutions [J].
Corti, Horacio R. ;
Appignanesi, Gustavo A. ;
Barbosa, Marcia C. ;
Bordin, J. Rafael ;
Calero, Carles ;
Camisasca, Gaia ;
Dolores Elola, M. ;
Franzese, Giancarlo ;
Gallo, Paola ;
Hassanali, Ali ;
Huang, Kai ;
Laria, Daniel ;
Menendez, Cintia A. ;
Montes de Oca, Joan M. ;
Paula Longinotti, M. ;
Rodriguez, Javier ;
Rovere, Mauro ;
Scherlis, Damian ;
Szleifer, Igal .
EUROPEAN PHYSICAL JOURNAL E, 2021, 44 (11)
[15]   Ice-binding proteins: a remarkable diversity of structures for stopping and starting ice growth [J].
Davies, Peter L. .
TRENDS IN BIOCHEMICAL SCIENCES, 2014, 39 (11) :548-555
[16]   Water structural transformation at molecular hydrophobic interfaces [J].
Davis, Joel G. ;
Gierszal, Kamil P. ;
Wang, Ping ;
Ben-Amotz, Dor .
NATURE, 2012, 491 (7425) :582-585
[17]   Antifreezing Hydroxyl Monolayer of Small Molecules on a Nanogold Surface [J].
Ding, Zhongxiang ;
Wang, Chao ;
Zhou, Baomei ;
Su, Mengke ;
Yang, Shixuan ;
Li, Yuzhu ;
Qu, Cheng ;
Liu, Honglin .
NANO LETTERS, 2022, 22 (13) :5307-5315
[18]   Adsorption of 2-mercaptopyridine and 4-mercaptopyridine on a silver surfaces investigated by SERS spectroscopy [J].
Do, Won Hui ;
Lee, Chul Jae ;
Kim, Dong Yeub ;
Jung, Maeng Joon .
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY, 2012, 18 (06) :2141-2146
[19]   Thin Layer Chromatography-Freeze Surface-Enhanced Raman Spectroscopy: A Powerful Tool for Monitoring Synthetic Reactions [J].
Fukunaga, Yu ;
Ogawa, Rintaro ;
Homma, Amane ;
Okada, Tetsuo .
CHEMISTRY-A EUROPEAN JOURNAL, 2023, 29 (39)
[20]   Freeze Surface-Enhanced Raman Scattering Coupled with Thin-Layer Chromatography: Pesticide Detection and Quantification Case [J].
Fukunaga, Yu ;
Okada, Tetsuo .
ANALYTICAL CHEMISTRY, 2022, 94 (39) :13507-13515