Identification and Characterization of Yeast Species Isolated from Cornus kousa Fruits in Japan

被引:0
|
作者
Otani, Norihiro [1 ]
Nguyen, Kieu D. M. [2 ]
Hirokawa, Atsushi [1 ]
Kanki, Yasuharu [3 ]
Yun, Hyun-Sik [4 ]
Maeda, Yoshiaki [5 ]
Gu, Wenchao [6 ]
Takahashi, Yoichiro [7 ]
Sugasawa, Takehito [3 ,8 ]
机构
[1] Univ Tsukuba, Coll Med, Sch Med & Hlth Sci, 1-1-1 Tennodai, Tsukuba 3058572, Japan
[2] Univ Tsukuba, Human Biol Program, 1-1-1 Tennodai, Tsukuba 3058572, Japan
[3] Univ Tsukuba, Inst Med, Dept Clin Med, Lab Clin Examinat & Sports Med, 1-1-1 Tennodai, Tsukuba 3058572, Japan
[4] Univ Tsukuba, Grad Sch Sci & Technol, 1-1-1 Tennodai, Tsukuba 3058572, Japan
[5] Univ Tsukuba, Inst Life & Environm Sci, 1-1-1 Tennodai, Tsukuba 3058572, Japan
[6] Univ Tsukuba, Dept Diagnost & Intervent Radiol, 1-1-1 Tennodai, Tsukuba 3058572, Japan
[7] Univ Tsukuba, Inst Med, Dept Legal Med, 1-1-1 Tennodai, Tsukuba 3058575, Japan
[8] Univ Tsukuba, Org Open Facil Initiat, Dept Sports Med Anal, Open Facil Network Off, 1-1-1 Tennodai, Tsukuba 3058572, Japan
来源
FERMENTATION-BASEL | 2024年 / 10卷 / 06期
关键词
Cornus kousa; Japanese dogwood; Yamaboushi; wild yeast species; Torulaspora delbrueckii; Pichia kluyveri; Saccharomyces cerevisiae; CELL-WALL; CONSTIPATION;
D O I
10.3390/fermentation10060288
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The Cornus kousa tree, which is of Asian origin, is often cultivated for ornamental purposes and used in traditional medicine. The tree produces sugar-rich fruits, which are potential habitats for natural yeasts. The identification of new yeast strains has many advantages for the industry and research. This study aimed to isolate and identify yeast species from C. kousa fruits and to understand their microbial ecology. Ripe and rotten fruits, which had fallen on the ground naturally, were collected and soaked in culture media, followed by plate spreading for colony growth. The morphological examination revealed three distinct colony types, including two from the ripe fruits and one from the rotten fruits. The analysis of the internal transcribed spacer 1 region indicated three yeast strains corresponding to the three colony types: Torulaspora delbrueckii and Pichia kluyveri from the ripe fruits and Saccharomyces cerevisiae from the rotten fruits. The metabolic characterizations demonstrated that all three yeasts efficiently consumed glucose and produced alcohol. S. cerevisiae exhibited the strongest fermentation ability and the highest growth rate. These findings showed that Cornus kousa fruit is a source of diverse yeast species, with distinct species associated with different states of fruit decomposition.
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页数:10
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