Circular RNA oligonucleotides: enzymatic synthesis and scaffolding for nanoconstruction

被引：1

作者：

Li, Shijie ^{[1
]}

Chu, Yanxin ^{[1
]}

Guo, Xin ^{[2
]}

Mao, Chengde ^{[3
]}

Xiao, Shou-Jun ^{[1
]}

机构：

[1] Nanjing Univ, Sch Chem & Chem Engn, Nanjing 210023, Jiangsu, Peoples R China

[2] Bruker Beijing Sci Technol Co Ltd, Beijing, Peoples R China

[3] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA

来源：

NANOSCALE HORIZONS | 2024年 / 9卷 / 10期

基金：

中国国家自然科学基金;

关键词：

EMERGING FIELD; DNA; DESIGN; NANOSTRUCTURES; MOLECULES; DELIVERY;

D O I：

10.1039/d4nh00236a

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We report the efficient synthesis of monomeric circular RNAs (circRNAs) in the size range of 16-44 nt with a novel DNA dumbbell splinting plus T4 DNA ligation strategy. Such a DNA dumbbell splinting strategy was developed by one group among ours recently for near-quantitative conversion of short linear DNAs into monomeric circular ones. Furthermore, using the 44 nt circRNA as scaffold strands, we constructed hybrid RNA:DNA and pure RNA:RNA double crossover tiles and their assemblies of nucleic acid nanotubes and flat arrays. Circular RNAs (similar to 16-44 nt) were enzymatically synthesized efficiently via a novel DNA dumbbell splinting strategy, further, the circular 44 nt RNA was used as scaffold strands to construct hybrid and pure RNA double crossover tiles and nanostructures.

引用

页码：1749 / 1755

页数：7

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