Circular RNA oligonucleotides: enzymatic synthesis and scaffolding for nanoconstruction

被引：1
作者：
Li, Shijie [1 ]
Chu, Yanxin [1 ]
Guo, Xin [2 ]
Mao, Chengde [3 ]
Xiao, Shou-Jun [1 ]
机构：
[1] Nanjing Univ, Sch Chem & Chem Engn, Nanjing 210023, Jiangsu, Peoples R China
[2] Bruker Beijing Sci Technol Co Ltd, Beijing, Peoples R China
[3] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA
来源：
NANOSCALE HORIZONS | 2024年 / 9卷 / 10期
基金：
中国国家自然科学基金;
关键词：
EMERGING FIELD; DNA; DESIGN; NANOSTRUCTURES; MOLECULES; DELIVERY;
D O I：
10.1039/d4nh00236a
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
We report the efficient synthesis of monomeric circular RNAs (circRNAs) in the size range of 16-44 nt with a novel DNA dumbbell splinting plus T4 DNA ligation strategy. Such a DNA dumbbell splinting strategy was developed by one group among ours recently for near-quantitative conversion of short linear DNAs into monomeric circular ones. Furthermore, using the 44 nt circRNA as scaffold strands, we constructed hybrid RNA:DNA and pure RNA:RNA double crossover tiles and their assemblies of nucleic acid nanotubes and flat arrays. Circular RNAs (similar to 16-44 nt) were enzymatically synthesized efficiently via a novel DNA dumbbell splinting strategy, further, the circular 44 nt RNA was used as scaffold strands to construct hybrid and pure RNA double crossover tiles and nanostructures.
引用
收藏
页码：1749 / 1755
页数：7
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