Structural insights into a functional unit from an immunogenic mollusk hemocyanin

被引:2
|
作者
Munoz, Sebastian M. [1 ]
Vallejos-Baccelliere, Gabriel [1 ]
Manubens, Augusto [2 ,3 ]
Salazar, Michelle L. [3 ]
Nascimento, Andrey F. Z. [4 ]
Tapia-Reyes, Patricio [5 ,6 ]
Meneses, Claudio [6 ,7 ,8 ,9 ]
Ambrosio, Andre L. B. [1 ,10 ]
Becker, Maria Ines [2 ,3 ]
Guixe, Victoria [1 ]
Castro-Fernandez, Victor [1 ]
机构
[1] Univ Chile, Fac Ciencias, Dept Biol, Lab Bioquim & Biol Mol, Santiago 780003, Chile
[2] Biosonda Corp, Dept Invest & Desarrollo, Santiago 7750629, Chile
[3] Fdn Ciencia & Tecnol Desarrollo FUCITED, Santiago 7750629, Chile
[4] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Synchrotron Light Lab LNLS, BR-13083970 Campinas, SP, Brazil
[5] Univ Santo Tomas, Fac Ciencias, Escuela Biotecnol, Santiago 8370003, Chile
[6] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Santiago 8331150, Chile
[7] Pontificia Univ Catolica Chile, Fac Agron & Sistemas Nat, Dept Fruticultura & Enol, Santiago 7820436, Chile
[8] Millennium Nucleus Dev Super Adaptable Plants MN S, Santiago 8331150, Chile
[9] Millennium Inst, Ctr Genome Regulat CRG, Santiago 8331150, Chile
[10] Univ Sao Paulo, Sao Carlos Inst Phys IFSC, BR-13563120 Sao Carlos, SP, Brazil
基金
巴西圣保罗研究基金会;
关键词
CRYO-EM STRUCTURE; CONCHOLEPAS HEMOCYANIN; N-GLYCANS; CARBOHYDRATE CHAINS; CRYSTAL-STRUCTURE; MOLECULAR-MODEL; SUBUNIT; REVEALS; KLH; INTERFACES;
D O I
10.1016/j.str.2024.02.018
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Mollusk hemocyanins, among the largest known proteins, are used as immunostimulants in biomedical and clinical applications. The hemocyanin of the Chilean gastropod Concholepas concholepas (CCH) exhibits unique properties, which makes it safe and effective for human immunotherapy, as observed in animal models of bladder cancer and melanoma, and dendritical cell vaccine trials. Despite its potential, the structure and amino acid sequence of CCH remain unknown. This study reports two sequence fragments of CCH, representing three complete functional units (FUs). We also determined the high -resolution (1.5 A & ring; ) X-ray crystal structure of an "FU -g type"from the CCHB subunit. This structure enables in-depth analysis of chemical interactions at the copper -binding center and unveils an unusual, truncated N-glycosylation pattern. These features are linked to eliciting more robust immunological responses in animals, offering insights into CCH's enhanced immunostimulatory properties and opening new avenues for its potential applications in biomedical research and therapies.
引用
收藏
页码:812 / 823.e4
页数:17
相关论文
共 50 条
  • [11] BIVALVE HEMOCYANIN - STRUCTURAL, FUNCTIONAL AND PHYLOGENETIC-RELATIONSHIPS
    MANGUM, CP
    MILLER, KI
    SCOTT, JL
    MORSE, MP
    VANHOLDE, KE
    AMERICAN ZOOLOGIST, 1986, 26 (04): : A57 - A57
  • [12] A functional hemocyanin in the leptostracan Nebalia:: Insights into molecular and arthropod phylogenies
    Vierthaler, L
    Ryan, M
    Terwilliger, NB
    INTEGRATIVE AND COMPARATIVE BIOLOGY, 2002, 42 (06) : 1328 - 1328
  • [13] Structural, Functional, and Immunogenic Insights on Cu,Zn Superoxide Dismutase Pathogenic Virulence Factors from Neisseria meningitidis and Brucella abortus
    Pratt, Ashley J.
    DiDonato, Michael
    Shin, David S.
    Cabelli, Diane E.
    Bruns, Cami K.
    Belzer, Carol A.
    Gorringe, Andrew R.
    Langford, Paul R.
    Tabatabai, Louisa B.
    Kroll, J. Simon
    Tainer, John A.
    Getzoff, Elizabeth D.
    JOURNAL OF BACTERIOLOGY, 2015, 197 (24) : 3834 - 3847
  • [14] Cytotoxic and apoptogenic properties of the hemocyanin derived from the marine mollusk Rapana thomasiana in vitro
    Genova-Kalou, P.
    Idakieva, K.
    Dundarova, D.
    Argirova, R.
    Alexandrova, R.
    Yotovska, K.
    Mohmmed, A.
    PLANTA MEDICA, 2008, 74 (09) : 1018 - 1018
  • [15] Functional unit of the Rapana thomasiana (Grosse) (marine snail, gastropod) hemocyanin
    Idakieva, K
    Stoeva, S
    Voelter, W
    Genov, N
    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1995, 112 (04): : 599 - 606
  • [16] Isolation, characterization, and monooxygenase activity of the minimal functional unit of octopus hemocyanin
    Shimokawa, Chizu
    Suzuki, Kenji
    Aono, Shigetoshi
    Itoh, Shinobu
    JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY, 2007, 12 (SUPPL 1): : S90 - S90
  • [17] Structure of a functional unit from the cylindrical wall of a gastropod hemocyanin-RtH2e from Rapana
    Guthöhrlein, E
    Perbandt, M
    Rypniewsky, W
    Stoeva, S
    Voelter, W
    Genov, N
    Betzel, C
    BIOPHYSICAL JOURNAL, 2002, 82 (01) : 316A - 317A
  • [18] A challenging insight on the structural unit 1 of molluscan Rapana venosa hemocyanin
    Dolashka-Angelova, Pavlina
    Stevanovic, Stefan
    Dolashki, Alexandar
    Devreese, Bart
    Tzvetkova, Bonka
    Voelter, Wolfgang
    Van Beeumen, Jozef
    Salvato, Benedetto
    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2007, 459 (01) : 50 - 58
  • [19] COMPLETE AMINO-ACID-SEQUENCE OF A FUNCTIONAL UNIT FROM A MOLLUSCAN HEMOCYANIN (HELIX-POMATIA)
    DREXEL, R
    SIEGMUND, S
    SCHNEIDER, HJ
    LINZEN, B
    GIELENS, C
    PREAUX, G
    LONTIE, R
    KELLERMANN, J
    LOTTSPEICH, F
    BIOLOGICAL CHEMISTRY HOPPE-SEYLER, 1987, 368 (06): : 617 - 635
  • [20] DIMERIC AND TETRAMERIC HEMOGLOBINS FROM THE MOLLUSK SCAPHARCA-INAEQUIVALVIS - STRUCTURAL AND FUNCTIONAL-PROPERTIES
    CHIANCONE, E
    VECCHINI, P
    VERZILI, D
    ASCOLI, F
    ANTONINI, E
    JOURNAL OF MOLECULAR BIOLOGY, 1981, 152 (03) : 577 - 592