Diverse bioactive secondary metabolites from Aspergillus terreus: antimicrobial, anticancer, and anti-SARS-CoV-2 activity studies

被引：1

作者：

Hamed, Abdelaaty ^{[1
]}

Abdel-Razek, Ahmed S. ^{[2
,3
]}

Abdelwahab, Ahmed B. ^{[4
]}

El Taweel, Ahmed ^{[5
]}

Gaballah, Mohamed ^{[5
]}

Sewald, Norbert ^{[2
,3
]}

Shaaban, Mohamed ^{[6
]}

机构：

[1] Al Azhar Univ, Fac Sci, Chem Dept, Cairo 11884, Egypt

[2] Bielefeld Univ, Fac Chem, Organ & Bioorgan Chem, D-33501 Bielefeld, Germany

[3] Inst Genet Engn & Biotechnol Res, Natl Res Ctr, Microbial Chem Dept, El Buhouth St 33, Dokki, Egypt

[4] Temisis Therapeut, 19 Ave Foret Haye, F-54500 Vandoeuvre Les Nancy, France

[5] Natl Res Ctr, Environm Res Div, Ctr Sci Excellence Influenza Virus, Giza 12622, Egypt

[6] Natl Res Ctr, Pharmaceut & Drug Ind Res Inst, Chem Nat Cpds Dept, El Buhouth St 33, Dokki 12622, Egypt

来源：

ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES | 2024年 / 79卷 / 11-12期

关键词：

marine Aspergillus terreus; bioactive metabolites; antimicrobial; cytotoxicity; anti-SARS-CoV-2; activities; BIOSYNTHESIS; DERIVATIVES;

D O I：

10.1515/znc-2024-0083

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Owing to its high interest as prolific source of diverse bioactive compounds referred in our previous research work, we have scaled-up the fermentation of the marine Aspergillus terreus LGO13 on a liquid culture medium to isolate and identify the very minor/further promising bioactive secondary metabolites and to study their antibacterial, cytotoxic, and antiviral properties. Twenty-three known bioactive metabolites, including the recently discovered microbial natural product N-benzoyl-tryptophan (1), were obtained herein. Their structures were determined using HR-ESI-MS 1D/2D NMR spectroscopy and data from the literature. The biological properties of the microbial extract and the resulting compounds were examined using a set of microorganisms, cervix carcinoma KB-3-1, nonsmall cell lung cancer (NSCLC) A549, and coronavirus (SARS-CoV-2), respectively. Molecular docking (MD) simulations were used to investigate the potential targets of the separated metabolites as anti-SARS-CoV-2 drugs. According to the current study, a viral protein that may be the target of anticovid drugs is a papain-like protease (PLpro), and chaetominine (2) appears to be a viable choice against this protein. We evaluated the antiviral efficacy of chaetominine (2), fumitremorgin C (6), and azaspirofuran A (9) against SARS-CoV-2 based on MD data. Chaetominine (2) and azaspirofuran A (9) displayed intermediate selectivity indices (SI = 6.6 and 3.2, respectively), while fumitremorgin C (6) displayed a high selectivity index (SI = 19.77). These findings show that fumitremorgin C has promising antiviral action against SARS-CoV-2.

引用

页码：361 / 369

页数：9