Femtosecond fiber delivery at 920 nm for two-photon microscopy

被引：0

作者：

Birkmeier, Konrad ^{[1
]}

Li, Yik Long ^{[1
]}

Roeser, Fabian ^{[1
]}

Herda, Robert ^{[1
]}

Lohr, Laura ^{[1
]}

Hofmann, Luisa ^{[1
]}

Wolfring, Bernhard ^{[1
]}

Haering, Reto ^{[1
]}

Tauser, Florian ^{[1
]}

机构：

[1] TOPTICA Photon AG, Lochhamer Schlag 19, D-82166 Graefelfing, Germany

来源：

MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES XXIV | 2024年 / 12847卷

关键词：

Femtosecond laser; multi-photon microscopy; two-photon microscopy; ultrafast fiber delivery; LASER;

D O I：

10.1117/12.3002446

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

We present a femtosecond laser system at 920 nm delivering ultrashort pulses via a hollow-core photonic bandgap fiber (HC-PBGF). The laser system is designed to simplify two-photon microscopy applications and can be used for miniaturized two-photon microscopes.1 While previously presented solutions have been tailored to a specific length and dispersion coefficient of the HC-PBGF,1 we now show a compact and flexible scheme for dispersion compensation which is compatible with a wide range of fiber types and lengths. In addition, this new approach fully maintains the capability of software-controlled dispersion compensation in the range from 0 to -40,000 fs(2) after the pulse delivery fiber. Hence, the dispersion of common two-photon microscopes can be pre-compensated in order to obtain compressed pulses at the sample plane. Our newly developed system displays excellent long-term fiber coupling stability under varying environmental conditions. It is capable of polarization-preserving femtosecond pulse delivery at 920 nm and reaches Watt-level power after the delivery fiber, making it suitable for in-vivo brain imaging of GCaMP in mouse models.

引用

页数：8

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