Superoxide Dismutase Premodulates Oxidative Stress in Plastids for Protection of Tobacco Plants from Cold Damage Ultrastructure Damage

被引:4
|
作者
Baranova, Ekaterina N. [1 ,2 ,3 ]
Kononenko, Neonila V. [1 ]
Lapshin, Pyotr V. [4 ]
Nechaeva, Tatiana L. [4 ]
Khaliluev, Marat R. [1 ,3 ]
Zagoskina, Natalia V. [4 ]
Smirnova, Elena A. [1 ,5 ,6 ]
Yuorieva, Natalya O. [4 ]
Raldugina, Galina N. [4 ]
Chaban, Inna A. [1 ]
Kurenina, Ludmila V. [1 ]
Gulevich, Alexander A. [1 ]
机构
[1] All Russia Res Inst Agr Biotechnol, Timiryazevskaya St 42, Moscow 127550, Russia
[2] Russian Acad Sci, NV Tsitsin Main Bot Garden, Moscow 127276, Russia
[3] Russian State Agr Univ, Moscow KA Timiryazev Agr Acad RSAU MTAA, Timiryazevskaya 49, Moscow 127434, Russia
[4] Russian Acad Sci, Timiryazev Inst Plant Physiol, Botanicheskaya St 35, Moscow 127276, Russia
[5] Lomonosov Moscow State Univ, Biol Fac, Leninskie Gory 1,Bldg 12, Moscow 119991, Russia
[6] MSU BIT Univ, Dept Biol, Shenzhen 518172, Peoples R China
关键词
superoxide dismutase; ultrastructure; plastid grana lamellae; chloroplast structural damage; Nicotiana tabacum L; ROS; OXYGEN SPECIES PRODUCTION; REACTIVE OXYGEN; SALT STRESS; ABIOTIC STRESS; ASCORBATE PEROXIDASE; ENHANCED TOLERANCE; POTATO PLANTS; PHOTOSYNTHETIC APPARATUS; PHENOLIC-COMPOUNDS; TOMATO PLANTS;
D O I
10.3390/ijms25105544
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
ROS-dependent induction of oxidative damage can be used as a trigger initiating genetically determined non-specific protection in plant cells and tissues. Plants are potentially able to withstand various specific (toxic, osmotic) factors of abiotic effects, but do not have sufficient or specific sensitivity to form an adequate effective response. In this work, we demonstrate one of the possible approaches for successful cold acclimation through the formation of effective protection of photosynthetic structures due to the insertion of the heterologous FeSOD gene into the tobacco genome under the control of the constitutive promoter and equipped with a signal sequence targeting the protein to plastid. The increased enzymatic activity of superoxide dismutase in the plastid compartment of transgenic tobacco plants enables them to tolerate the oxidative factor of environmental stresses scavenging ROS. On the other hand, the cost of such resistance is quite high and, when grown under normal conditions, disturbs the arrangement of the intrachloroplastic subdomains leading to the modification of stromal thylakoids, probably significantly affecting the photosynthesis processes that regulate the efficiency of photosystem II. This is partially compensated for by the fact that, at the same time, under normal conditions, the production of peroxide induces the activation of ROS detoxification enzymes. However, a violation of a number of processes, such as the metabolism of accumulation, and utilization and transportation of sugars and starch, is significantly altered, which leads to a shift in metabolic chains. The expected step for further improvement of the applied technology could be both the use of inducible promoters in the expression cassette, and the addition of other genes encoding for hydrogen peroxide-scavenging enzymes in the genetic construct that are downstream in the metabolic chain.
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页数:24
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