Development of a tobramycin-loaded calcium alginate microsphere/chitosan composite sponge with antibacterial effects as a wound dressing

被引:0
作者
Ma, Ruixia [1 ,2 ]
Shi, Xingyan [1 ,3 ]
Wang, Xiaoyan [1 ,3 ]
Si, Chenchen [4 ]
Gong, Yuwei [1 ,3 ]
Jian, Wei [1 ,3 ]
Zhou, Chen [1 ,3 ]
Yang, Hui [1 ,3 ]
Xu, Lihua [5 ]
Zhang, Hualin [1 ,3 ]
机构
[1] Ningxia Med Univ, Coll Stomatol, Yinchuan 750004, Peoples R China
[2] Third Peoples Hosp Yinchuan, Dept Stomatol, Yinchuan 750004, Peoples R China
[3] Ningxia Med Univ, Ningxia Prov Key Lab Oral Dis Res, Yinchuan 750004, Peoples R China
[4] Ningxia Med Univ, Gen Hosp, Yinchuan 750004, Peoples R China
[5] Wenzhou Med Univ, Affiliated Hosp 1, Dept Gen Med, Wenzhou 25000, Peoples R China
基金
中国国家自然科学基金;
关键词
composite sponge; wound dressing; antibacterial effect; tobramycin; sodium alginate; chitosan; CARBOXYMETHYL CHITOSAN; HYDROGEL;
D O I
10.1088/1748-605X/ad525e
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Traditional dressings exhibit several disadvantages, as they frequently lead to bacterial infections, cause severe tissue adhesion and perform a relatively single function. Therefore, in this study, a composite sponge dressing with antibacterial properties and excellent physicochemical properties was developed. Six groups of tobramycin-loaded calcium alginate microspheres were prepared by changing the amount of tobramycin added, and the optimal group was selected. Then, seven groups of tobramycin-loaded calcium alginate microsphere/chitosan composite sponges were fabricated via a solvent blending process and a freeze-drying method. The surface morphology, physicochemical properties, in vitro degradation properties, in vitro drug release properties, antibacterial properties and cytotoxicity of the composite sponges were examined. Group 3.0 contained the best microspheres with the largest drug loading capacity, good swelling performance and cumulative drug release rate, obvious and sustained antibacterial activity, and good cytocompatibility. The tobramycin-loaded calcium alginate microsphere/chitosan composite sponges exhibited three-dimensional porous structures, and their porosity, swelling rate, water absorption and water retention rates and water vapor transmission rate met the standards needed for an ideal dressing. The comprehensive performance of the sponge was best when 20 mg of drug-loaded microspheres was added (i.e. group 20). The cumulative drug release rate of the sponge was 29.67 +/- 4.14% at 7 d, the diameters of the inhibition zones against the three bacteria were greater than 15 mm, and L929 cell proliferation was promoted. These results demonstrated that the tobramycin-loaded calcium alginate microsphere/chitosan composite sponge with 20 mg of tobramycin-loaded microspheres shows promise as a dressing for infected wounds.
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页数:18
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