Occurrence of recreational water quality monitoring general fecal indicator bacteria and fecal source identification genetic markers in gray seal scat

被引:0
作者
Paar III, Jack [1 ]
Willis, Jessica R. [2 ]
Sette, Lisa [3 ]
Wood, Stephanie A. [4 ]
Bogomolni, Andrea [5 ]
Dulac, Monique [1 ]
Sivaganesan, Mano [2 ]
Shanks, Orin C. [2 ]
机构
[1] US EPA, New England Reg Lab, North Chelmsford, MA 01863 USA
[2] US EPA, Off Res & Dev, Ctr Environm Measurement & Modeling, Cincinnati, OH 45268 USA
[3] Ctr Coastal Studies, 5 Holway Ave, Provincetown, MA 02657 USA
[4] Univ Massachusetts, Biol Dept, 100 Morrissey Blvd, Boston, MA 02125 USA
[5] Massachusetts Maritime Acad, Marine Sci Safety & Environm Protect, 101 Acad Dr, Buzzards Bay, MA 02532 USA
关键词
Fecal source identification; Fecal indicators; Water quality; Marine mammals; qPCR; QUANTITATIVE PCR ASSAY; ESCHERICHIA-COLI; RIBOSOMAL-RNA; HALICHOERUS-GRYPUS; NONPOINT-SOURCE; HARBOR SEAL; FRESH-WATER; GREY SEALS; TIME; POLLUTION;
D O I
10.1016/j.scitotenv.2024.173220
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The number of gray seals (Halichoerus grypus) observed along the United States Northwest Atlantic region has been increasing for decades. These colonial animals often haul-out on beaches seasonally in numbers ranging from a few individuals to several thousands. While these larger aggregations are an important part of gray seal behavior, there is public concern that haul-outs could lead to large amounts of fecal waste in recreational areas, potentially resulting in beach closures. Yet, data to confirm whether these animals contribute to beach closures is lacking and minimal information is available on the occurrence of key water quality monitoring genetic markers in gray seal scat. This study evaluates the concentration of E. coli (EC23S857), enterococci (Entero1a), and fecal Bacteroidetes (GenBac3) as well as six fecal source identification genetic markers (HF183/BacR287, HumM2, CPQ_056, Rum2Bac, DG3, and GFD) measured by qPCR in 48 wild gray seal scat samples collected from two haul-out areas in Cape Cod (Massachusetts, U.S.A.). Findings indicate that FIB genetic markers are shed in gray seal scat at significantly different concentrations with the Entero1a genetic marker exhibiting the lowest average concentration (-0.73 log(10) estimated mean copies per nanogram of DNA). In addition, systematic testing of scat samples demonstrated that qPCR assays targeting host-associated genetic markers indicative of human, ruminant, and canine fecal pollution sources remain highly specific in waters frequented by gray seals (>97 % specificity).
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页数:10
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