RNA sequencing of formalin fixed paraffin-embedded heart tissue provides transcriptomic information about chemotherapy-induced cardiotoxicity

被引：1
作者：
Todorova, Valentina K. [1 ,2 ]
Bauer, Michael A. [3 ]
Azhar, Gohar [2 ]
Wei, Jeanne Y. [2 ]
机构：
[1] Univ Arkansas Med Sci, Div Hematol Oncol, 4301 W Markham Str 508, Little Rock, AR 72205 USA
[2] Univ Arkansas Med Sci, Dept Geriatr, Little Rock, AR USA
[3] Univ Arkansas Med Sci, Dept Biomed Informat, Little Rock, AR USA
来源：
PATHOLOGY RESEARCH AND PRACTICE | 2024年 / 257卷
关键词：
RNA sequencing; Gene expression; FFPE; Doxorubicin; Cardiotoxicity; GENE-EXPRESSION; DOXORUBICIN; INFLAMMATION; MECHANISMS; CARDIOMYOPATHY; BIOMARKERS; FAILURE; DISEASE;
D O I：
10.1016/j.prp.2024.155309
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
Gene expression of formalin-fixed paraffin -embedded (FFPE) tissue may serve for molecular studies on cardiovascular diseases. Chemotherapeutics, such as doxorubicin (DOX) may cause heart injury, but the mechanisms of these side effects of DOX are not well understood. This study aimed to investigate whether DOX-induced gene expression in archival FFPE heart tissue in experimental rats would correlate with the gene expression in freshfrozen heart tissue by applying RNA sequencing technology. The results showed RNA from FFPE samples was degraded, resulting in a lower number of uniquely mapped reads. However, DOX-induced differentially expressed genes in FFPE were related to molecular mechanisms of DOX-induced cardiotoxicity, such as inflammation, calcium binding, endothelial dysfunction, senescence, and cardiac hypertrophy signaling. Our data suggest that, despite the limitations, RNA sequencing of archival FFPE heart tissue supports utilizing FFPE tissues from retrospective studies on cardiovascular disorders, including DOX-induced cardiotoxicity.
引用
收藏
页数：7
相关论文
共 66 条
[1]   European Society of Cardiology quality indicators for the management of patients with ventricular arrhythmias and the prevention of sudden cardiac death Developed in collaboration with the European Heart Rhythm Association of the European Society of Cardiology [J].
Aktaa, Suleman ;
Tzeis, Stylianos ;
Gale, Chris P. ;
Ackerman, Michael J. ;
Arbelo, Elena ;
Behr, Elijah R. ;
Crotti, Lia ;
d'Avila, Andre ;
de Chillou, Christian ;
Denekel, Thomas ;
Figueiredo, Marcio ;
Friede, Tim ;
Leclercq, Christophe ;
Merino, Jose L. ;
Semsarian, Chris ;
Verstrael, Axel ;
Zeppenfeld, Katja ;
Tfelt-Hansen, Jacob ;
Reichlin, Tobias .
EUROPACE, 2023, 25 (01) :199-210
[2]   Protocol Identification of regulatory links between transcription and RNA processing with long-read sequencing [J].
Alfonso-Gonzalez, Carlos ;
Arrigoni, Laura ;
Ozbulut, Hasan Can ;
Falk, Stefanie ;
Boenisch, Ulrike ;
Hilgers, Valerie .
STAR PROTOCOLS, 2023, 4 (04)
[3]   Pathology of late-onset anthracycline cardiomyopathy [J].
Bernaba, Bob N. ;
Chan, Jessica B. ;
Lai, Chi K. ;
Fishbein, Michael C. .
CARDIOVASCULAR PATHOLOGY, 2010, 19 (05) :308-311
[4]   Anthracycline-Induced Cardiotoxicity: Causes, Mechanisms, and Prevention [J].
Bhagat, Anchit ;
Kleinerman, Eugenie S. .
CURRENT ADVANCES IN OSTEOSARCOMA: CLINICAL PERSPECTIVES: PAST, PRESENT AND FUTURE, 2ND EDITION, 2020, 1257 :181-192
[5]  
BILLINGHAM ME, 1978, CANCER TREAT REP, V62, P865
[6]   Autophagy and Mitophagy in Cardiovascular Disease [J].
Bravo-San Pedro, Jose Manuel ;
Kroemer, Guido ;
Galluzzi, Lorenzo .
CIRCULATION RESEARCH, 2017, 120 (11) :1812-1824
[7]   Association of Anthracycline-Related Cardiac Histological Lesions With NADPH Oxidase Functional Polymorphisms [J].
Cascales, Almudena ;
Pastor-Quirante, Francisco ;
Sanchez-Vega, Beatriz ;
Luengo-Gil, Gines ;
Corral, Javier ;
Ortuno-Pacheco, Guzman ;
Vicente, Vicente ;
Ayala de la Pena, Francisco .
ONCOLOGIST, 2013, 18 (04) :446-453
[8]   Management of cardiac disease in cancer patients throughout oncological treatment: ESMO consensus recommendations [J].
Curigliano, G. ;
Lenihan, D. ;
Fradley, M. ;
Ganatra, S. ;
Barac, A. ;
Blaes, A. ;
Herrmann, J. ;
Porter, C. ;
Lyon, A. R. ;
Lancellotti, P. ;
Patel, A. ;
DeCara, J. ;
Mitchell, J. ;
Harrison, E. ;
Moslehi, J. ;
Witteles, R. ;
Calabro, M. G. ;
Orecchia, R. ;
de Azambuja, E. ;
Zamorano, J. L. ;
Krone, R. ;
Iakobishvili, Z. ;
Carver, J. ;
Armenian, S. ;
Ky, B. ;
Cardinale, D. ;
Cipolla, C. M. ;
Dent, S. ;
Jordan, K. .
ANNALS OF ONCOLOGY, 2020, 31 (02) :171-190
[9]   GPR91 Receptor Mediates Protection against Doxorubicin-Induced Cardiotoxicity without Altering Its Anticancer Efficacy. An In Vitro Study on H9C2 Cardiomyoblasts and Breast Cancer-Derived MCF-7 Cells [J].
Dallons, Matthieu ;
Alpan, Esma ;
Schepkens, Corentin ;
Tagliatti, Vanessa ;
Colet, Jean-Marie .
CELLS, 2020, 9 (10) :1-19
[10]   DAVID: Database for annotation, visualization, and integrated discovery [J].
Dennis, G ;
Sherman, BT ;
Hosack, DA ;
Yang, J ;
Gao, W ;
Lane, HC ;
Lempicki, RA .
GENOME BIOLOGY, 2003, 4 (09)
1234567