Evolution Characterization and Pathogenicity of an NADC34-like PRRSV Isolated from Inner Mongolia, China

被引：5

作者：

Zhao, Hong-Zhe ^{[1
]}

Liu, Chun-Yu ^{[2
]}

Meng, Hai ^{[1
]}

Sun, Cheng-Long ^{[1
]}

Yang, Hong-Wen ^{[1
]}

Wang, Hao ^{[1
]}

Zou, Jian ^{[1
]}

Li, Peng ^{[1
]}

Han, Feng-Ye ^{[1
]}

Qi, Gen ^{[1
]}

Zhang, Yang ^{[1
]}

Lin, Bing-Bing ^{[1
]}

Liu, Chuang ^{[1
]}

Chen, Meng-Meng ^{[1
]}

Zhang, Pan-Ling ^{[1
]}

Chen, Xiao-Dong ^{[1
]}

Zhang, Yi-Di ^{[1
]}

Song, Qian-Jin ^{[3
]}

Wen, Yong-Jun ^{[1
]}

Wang, Feng-Xue ^{[1
]}

机构：

[1] Inner Mongolia Agr Univ, Minist Agr, Coll Vet Med, Key Lab Clin Diag & Treatment Anim Dis, Hohhot 010018, Peoples R China

[2] Inner Mongolia Med Univ, Med Expt Ctr, Hohhot 010018, Peoples R China

[3] Yinchuan Anim Husb Technol Extens Serv Ctr, Yinchuan 750000, Peoples R China

来源：

VIRUSES-BASEL | 2024年 / 16卷 / 05期

关键词：

porcine reproductive and respiratory syndrome virus; genomic characterization; NADC34-like; pathogenicity; RESPIRATORY-SYNDROME-VIRUS; MYSTERY SWINE DISEASE; EPIDEMIOLOGY; INFECTION;

D O I：

10.3390/v16050683

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Porcine reproductive and respiratory syndrome virus (PRRSV) is a pathogen that causes severe abortions in sows and high piglet mortality, resulting in huge economic losses to the pig industry worldwide. The emerging and novel PRRSV isolates are clinically and biologically important, as there are likely recombination and pathogenic differences among PRRSV genomes. Furthermore, the NADC34-like strain has become a major epidemic strain in some parts of China, but the characterization and pathogenicity of the latest strain in Inner Mongolia have not been reported in detail. In this study, an NADC34-like strain (CHNMGKL1-2304) from Tongliao City, Inner Mongolia was successfully isolated and characterized, and confirmed the pathogenicity in pigs. The phylogenetic tree showed that this strain belonged to sublineage 1.5 and had high homology with the strain JS2021NADC34. There is no recombination between CHNMGKL1-2304 and any other domestic strains. Animal experiments show that the CHNMGKL1-2304 strain is moderately virulent to piglets, which show persistent fever, weight loss and high morbidity but no mortality. The presence of PRRSV nucleic acids was detected in both blood, tissues, nasal and fecal swabs. In addition, obvious pathological changes and positive signals were observed in lung, lymph node, liver and spleen tissues when subjected to hematoxylin-eosin (HE) staining and immunohistochemistry (IHC). This report can provide a basis for epidemiological investigations and subsequent studies of PRRSV.

引用

页数：16

共 33 条

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