CRISPR-based genetic screens advance cancer immunology

被引：3
作者：
Cao, Yuanfang [1 ]
Li, Xueting [1 ]
Pan, Yumu [1 ]
Wang, Huahe [1 ]
Yang, Siyu [1 ]
Hong, Lingjuan [1 ]
Ye, Lupeng [1 ]
机构：
[1] Nanjing Univ, Inst Modern Biol, Nanjing 210008, Peoples R China
来源：
SCIENCE CHINA-LIFE SCIENCES | 2024年 / 67卷 / 12期
关键词：
gene editing; CRISPR/Cas; genetic screening; cancer immunotherapy; immune cells; HUMAN-CELLS; T-CELLS; TRANSCRIPTIONAL ACTIVATION; ACQUIRED-RESISTANCE; GENOMIC DNA; RNA; TARGET; IMMUNOTHERAPY; ORGANOIDS; KNOCKOUT;
D O I：
10.1007/s11427-023-2571-0
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
CRISPR technologies have revolutionized research areas ranging from fundamental science to translational medicine. CRISPR-based genetic screens offer a powerful platform for unbiased screening in various fields, such as cancer immunology. Immune checkpoint blockade (ICB) therapy has been shown to strongly affect cancer treatment. However, the currently available ICBs are limited and do not work in all cancer patients. Pooled CRISPR screens enable the identification of previously unknown immune regulators that can regulate T-cell activation, cytotoxicity, persistence, infiltration into tumors, cytokine secretion, memory formation, T-cell metabolism, and CD4+ T-cell differentiation. These novel targets can be developed as new immunotherapies or used with the current ICBs as new combination therapies that may yield synergistic efficacy. Here, we review the progress made in the development of CRISPR technologies, particularly technological advances in CRISPR screens and their application in novel target identification for immunotherapy.
引用
收藏
页码：2554 / 2562
页数：9
相关论文
共 115 条
[61]   Computational Methods for Analysis of Large-Scale CRISPR Screens [J].
Lin, Xueqiu ;
Chemparathy, Augustine ;
La Russa, Marie ;
Daley, Timothy ;
Qi, Lei S. .
ANNUAL REVIEW OF BIOMEDICAL DATA SCIENCE, VOL 3, 2020, 2020, 3 :137-162
[62]   CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells [J].
Liu, S. John ;
Horlbeck, Max A. ;
Cho, Seung Woo ;
Birk, Harjus S. ;
Malatesta, Martina ;
He, Daniel ;
Attenello, Frank J. ;
Villalta, Jacqueline E. ;
Cho, Min Y. ;
Chen, Yuwen ;
Mandegar, Mohammad A. ;
Olvera, Michael P. ;
Gilbert, Luke A. ;
Conklin, Bruce R. ;
Chang, Howard Y. ;
Weissman, Jonathan S. ;
Lim, Daniel A. .
SCIENCE, 2017, 355 (6320)
[63]   CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity [J].
Long, Lingyun ;
Wei, Jun ;
Lim, Seon Ah ;
Raynor, Jana L. ;
Shi, Hao ;
Connelly, Jon P. ;
Wang, Hong ;
Guy, Cliff ;
Xie, Boer ;
Chapman, Nicole M. ;
Fu, Guotong ;
Wang, Yanyan ;
Huang, Hongling ;
Su, Wei ;
Saravia, Jordy ;
Risch, Isabel ;
Wang, Yong-Dong ;
Li, Yuxin ;
Niu, Mingming ;
Dhungana, Yogesh ;
Anil, K. C. ;
Zhou, Peipei ;
Vogel, Peter ;
Yu, Jiyang ;
Pruett-Miller, Shondra M. ;
Peng, Junmin ;
Chi, Hongbo .
NATURE, 2021, 600 (7888) :308-+
[64]   A Genome-wide CRISPR Screen Reveals a Role for the Non-canonical Nucleosome-Remodeling BAF Complex in Foxp3 Expression and Regulatory T Cell Function [J].
Loo, Chin-San ;
Gatchalian, Jovylyn ;
Liang, Yuqiong ;
Leblanc, Mathias ;
Xie, Mingjun ;
Ho, Josephine ;
Venkatraghavan, Bhargav ;
Hargreaves, Diana C. ;
Zheng, Ye .
IMMUNITY, 2020, 53 (01) :143-+
[65]   Safety and efficacy of gene transfer for Leber's congenital amaurosis [J].
Maguire, Albert M. ;
Simonelli, Francesca ;
Pierce, Eric A. ;
Pugh, Edward N., Jr. ;
Mingozzi, Federico ;
Bennicelli, Jeannette ;
Banfi, Sandro ;
Marshall, Kathleen A. ;
Testa, Francesco ;
Surace, Enrico M. ;
Rossi, Settimio ;
Lyubarsky, Arkady ;
Arruda, Valder R. ;
Konkle, Barbara ;
Stone, Edwin ;
Sun, Junwei ;
Jacobs, Jonathan ;
Dell'Osso, Lou ;
Hertle, Richard ;
Ma, Jian-xing ;
Redmond, T. Michael ;
Zhu, Xiaosong ;
Hauck, Bernd ;
Zelenaia, Olga ;
Shindler, Kenneth S. ;
Maguire, Maureen G. ;
Wright, J. Fraser ;
Volpe, Nicholas J. ;
McDonnell, Jennifer Wellman ;
Auricchio, Alberto ;
High, Katherine A. ;
Bennett, Jean .
NEW ENGLAND JOURNAL OF MEDICINE, 2008, 358 (21) :2240-2248
[66]  
Mali P, 2013, NAT METHODS, V10, P957, DOI [10.1038/NMETH.2649, 10.1038/nmeth.2649]
[67]   RNA-Guided Human Genome Engineering via Cas9 [J].
Mali, Prashant ;
Yang, Luhan ;
Esvelt, Kevin M. ;
Aach, John ;
Guell, Marc ;
DiCarlo, James E. ;
Norville, Julie E. ;
Church, George M. .
SCIENCE, 2013, 339 (6121) :823-826
[68]   In vivo CRISPR screening identifies Ptpn2 as a cancer immunotherapy target [J].
Manguso, Robert T. ;
Pope, Hans W. ;
Zimmer, Margaret D. ;
Brown, Flavian D. ;
Yates, Kathleen B. ;
Miller, Brian C. ;
Collins, Natalie B. ;
Bi, Kevin ;
Lafleur, Martin W. ;
Juneja, Vikram R. ;
Weiss, Sarah A. ;
Lo, Jennifer ;
Fisher, David E. ;
Miao, Diana ;
Van Allen, Eliezer ;
Root, David E. ;
Sharpe, Arlene H. ;
Doench, John G. ;
Haining, W. Nicholas .
NATURE, 2017, 547 (7664) :413-+
[69]   Massively parallel base editing to map variant effects in human hematopoiesis [J].
Martin-Rufino, Jorge D. ;
Castano, Nicole ;
Pang, Michael ;
Grody, Emanuelle I. ;
Joubran, Samantha ;
Caulier, Alexis ;
Wahlster, Lara ;
Li, Tongqing ;
Qiu, Xiaojie ;
Riera-Escandell, Anna Maria ;
Newby, Gregory A. ;
Al'Khafaji, Aziz ;
Chaudhary, Santosh ;
Black, Susan ;
Weng, Chen ;
Munson, Glen ;
Liu, David R. ;
Wlodarski, Marcin W. ;
Sims, Kacie ;
Oakley, Jamie H. ;
Fasano, Ross M. ;
Xavier, Ramnik J. ;
Lander, Eric S. ;
Klein, Daryl E. ;
Sankaran, Vijay G. .
CELL, 2023, 186 (11) :2456-+
[70]   Single-Dose Gene-Replacement Therapy for Spinal Muscular Atrophy [J].
Mendell, J. R. ;
Al-Zaidy, S. ;
Shell, R. ;
Arnold, W. D. ;
Rodino-Klapac, L. R. ;
Prior, T. W. ;
Lowes, L. ;
Alfano, L. ;
Berry, K. ;
Church, K. ;
Kissel, J. T. ;
Nagendran, S. ;
L'Italien, J. ;
Sproule, D. M. ;
Wells, C. ;
Cardenas, J. A. ;
Heitzer, M. D. ;
Kaspar, A. ;
Corcoran, S. ;
Braun, L. ;
Likhite, S. ;
Miranda, C. ;
Meyer, K. ;
Foust, K. D. ;
Burghes, A. H. M. ;
Kaspar, B. K. .
NEW ENGLAND JOURNAL OF MEDICINE, 2017, 377 (18) :1713-1722
234567891011