Daphnetin inhibits invasion and migration of LM8 murine osteosarcoma cells by decreasing RhoA and Cdc42 expression

被引：38

作者：

Fukuda, Hiroki ^{[1
]}

Nakamura, Seikou ^{[2
]}

Chisaki, Yugo ^{[3
]}

Takada, Tetsuya ^{[1
]}

Toda, Yuki ^{[4
]}

Murata, Hiroaki ^{[5
,6
]}

Itoh, Kazuyuki ^{[7
]}

Yano, Yoshitaka ^{[3
]}

Takata, Kazuyuki ^{[1
]}

Ashihara, Eishi ^{[1
]}

机构：

[1] Kyoto Pharmaceut Univ, Dept Clin & Translat Physiol, Kyoto 607, Japan

[2] Kyoto Pharmaceut Univ, Dept Pharmacognosy, Kyoto 607, Japan

[3] Kyoto Pharmaceut Univ, Educ & Res Ctr Clin Pharm, Kyoto 607, Japan

[4] Kyoto Pharmaceut Univ, Dept Med Chem, Kyoto 607, Japan

[5] Kyoto Prefectural Univ Med, Grad Sch Med Sci, Dept Orthoped, Kyoto, Japan

[6] Matsushita Mem Hosp, Dept Orthoped Surg, Osaka, Japan

[7] Osaka Med Ctr Canc & Cardiovasc Dis, Dept Biol, Osaka, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2016年 / 471卷 / 01期

关键词：

LM8; Metastasis; daphnetin; Rho family small GTP-binding proteins; Stress fiber; Filopodia; SMALL INTERFERING RNA; ACTIN STRESS FIBERS; COUMARIN DERIVATIVES; PROTEIN-KINASE; TUMOR-CELLS; GROWTH; DIFFERENTIATION; METASTASIS; ESCULETIN; ARTHRITIS;

D O I：

10.1016/j.bbrc.2016.01.179

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Daphnetin, 7,8-dihydroxycoumarin, present in main constituents of Daphne odora var. marginatai, has multiple pharmacological activities including anti-proliferative effects in cancer cells. In this study, using a Transwell system, we showed that daphnetin inhibited invasion and migration of highly metastatic murine osteosarcoma LM8 cells in a dose-dependent manner. Following treatment by daphnetin, cells that penetrated the Transwell membrane were rounder than non-treated cells. Immunofluorescence analysis revealed that daphnetin decreased the numbers of intracellular stress fibers and filopodia. Moreover, daphnetin treatment dramatically decreased the expression levels of RhoA and Cdc42. In summary, the dihydroxycoumarin derivative daphnetin inhibits the invasion and migration of LM8 cells, and therefore represents a promising agent for use against metastatic cancer. (C) 2016 Elsevier Inc. All rights reserved.

引用

页码：63 / 67

页数：5

共 24 条

[1] Differential effects of esculetin and daphnetin on in vitro cell proliferation and in vivo estrogenicity [J].

Alejandro Jimenez-Orozco, Fausto ;

Roman Rosales, Ana Alejandra ;

Vega-Lopez, Armando ;

Dominguez-Lopez, Maria Lilia ;

Juana Garcia-Mondragon, Ma ;

Maldonado-Espinoza, Amelia ;

Lemini, Cristina ;

Mendoza-Patino, Nicandro ;

Jose Mandoki, Juan .

EUROPEAN JOURNAL OF PHARMACOLOGY, 2011, 668 (1-2) :35-41

[2]

Asai T, 1998, INT J CANCER, V76, P418, DOI 10.1002/(SICI)1097-0215(19980504)76:3<418::AID-IJC21>3.3.CO

[3]

2-E

[4] β-Catenin Small Interfering RNA Successfully Suppressed Progression of Multiple Myeloma in a Mouse Model [J].

Ashihara, Eishi ;

Kawata, Eri ;

Nakagawa, Yoko ;

Shimazaski, Chihiro ;

Kuroda, Junya ;

Taniguchi, Kyoko ;

Uchiyama, Hitoji ;

Tanaka, Ruriko ;

Yokota, Asumi ;

Takeuchi, Miki ;

Kamitsuji, Yuri ;

Inaba, Tohru ;

Taniwaki, Masafumi ;

Kimura, Shinya ;

Maekawa, Taira .

CLINICAL CANCER RESEARCH, 2009, 15 (08) :2731-2738

[5] Daphnetin induced differentiation of human renal carcinoma cells and its mediation by p38 mitogen-activated protein kinase [J].

Finn, GJ ;

Creaven, BS ;

Egan, DA .

BIOCHEMICAL PHARMACOLOGY, 2004, 67 (09) :1779-1788

[6] In vitro cytotoxic potential and mechanism of action of selected coumarins, using human renal cell lines [J].

Finn, GJ ;

Kenealy, E ;

Creaven, BS ;

Egan, DA .

CANCER LETTERS, 2002, 183 (01) :61-68

[7] Natural and synthetic coumarin derivatives with anti-inflammatory/antioxidant activities [J].

Fylaktakidou, KC ;

Hadjipavlou-Litina, DJ ;

Litinas, KE ;

Nicolaides, DN .

CURRENT PHARMACEUTICAL DESIGN, 2004, 10 (30) :3813-3833

[8]

Huang F, 2006, BIOMED ENVIRON SCI, V19, P367

[9] Administration of PLK-1 small interfering RNA with atelocollagen prevents the growth of liver metastases of lung cancer [J].

Kawata, Eri ;

Ashihara, Eishi ;

Kimura, Shinya ;

Takenaka, Kazumasa ;

Sato, Kiyoshi ;

Tanaka, Ruriko ;

Yokota, Asumi ;

Kamitsuji, Yuri ;

Takeuchi, Miki ;

Kuroda, Junya ;

Tanaka, Fumihiro ;

Yoshikawa, Toshikazu ;

Maekawa, Taira .

MOLECULAR CANCER THERAPEUTICS, 2008, 7 (09) :2904-2912

[10] Antitumor and antimetastatic actions of dihydroxycoumarins (esculetin or fraxetin) through the inhibition of M2 macrophage differentiation in tumor-associated macrophages and/or G1 arrest in tumor cells [J].

Kimura, Yoshiyuki ;

Sumiyoshi, Maho .

EUROPEAN JOURNAL OF PHARMACOLOGY, 2015, 746 :115-125

← 1 2 3 →