Three-dimensional lung parenchyma model for studies of Aspergillus fumigatus infection and antifungal treatment

被引：0

作者：

Fortes, Bruna Nakanishi ^{[1
]}

Wirth, Fernanda ^{[1
]}

dos Santos, Aline Martins ^{[2
]}

Chorilli, Marlus ^{[2
]}

Freitas, Vanessa Morais ^{[1
]}

Farias, Jennifer ^{[3
]}

Chambergo, Felipe S. ^{[3
]}

Dantas, Viviane Abreu Nunes C. ^{[3
]}

Ishida, Kelly ^{[1
]}

机构：

[1] Univ Sao Paulo, Inst Biomed Sci, Prof Lineu Prestes Ave 1374, BR-05508000 Sao Paulo, Brazil

[2] Sao Paulo State Univ Jau Highway, Sch Pharmaceut Sci, UNESP, Km 1, BR-14801902 Araraquara, Brazil

[3] Univ Sao Paulo, Sch Arts Sci & Humanities, Arlindo Bettio Ave 1000, BR-03828000 Sao Paulo, Brazil

来源：

FUTURE MICROBIOLOGY | 2024年 / 19卷 / 14期

基金：

巴西圣保罗研究基金会;

关键词：

amphotericin B; antifungals; Aspergillus fumigatus; biofilm; lung epithelial cell; scaffold; three-dimensional cell culture; voriconazole; PROLIFERATION; HYDROGELS;

D O I：

10.1080/17460913.2024.2371926

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Aim: This work aims to standardize the three-dimensional hydroxyethyl-alginate-gelatin (HAG) scaffold as a model to evaluate Aspergillus fumigatus biofilm and antifungal treatments. Methods: The scaffold was characterized by physical, rheological and microscopic analyses; the antibiofilm action was evaluated by determination of cfu and metabolic activity. Results: The scaffold was non-toxic showing stability in aqueous media, swelling capacity, elasticity and had homogeneously distributed pores averaging 190 mu m. The A. fumigatus biofilm established itself very well on the scaffold and treatment with amphotericin B and voriconazole reduced viable cells and metabolic activity. Conclusion: The HAG scaffold proved to be a model to mimic lung parenchyma, suitable for establishing a 3D biofilm culture of A. fumigatus and evaluating the efficacy of antifungals.

引用

页码：1203 / 1216

页数：14

共 33 条

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